Kobayashi Y, Inoue Y, Shibata K
Biochim Biophys Acta. 1976 Jan 15;423(1):80-90. doi: 10.1016/0005-2728(76)90102-x.
The treatment of spinach chloroplasts with p-nitrothiophenol in the light at acidic and neutral pH'S caused specific inhibition of the Photosystem II activity, whereas the same treatment in the dark did not affect the activity at all. The photosystem I activity was not inhibited by p-nitrothiophenol both in the light and in the dark. The inhibition was accompanied by changes of fluorescence from chloroplasts. As observed at room temperature, the 685-nm band was lowered by the p-nitrothiophenol treatment in the light and, at liquid nitrogen temperature, the relative height of the 695-nm band to the 685-nm band increased and the 695-nm band shifted to longer wavelengths. The action spectra for these effects of p-nitrothiophenol on the activity and fluorescence showed a peak at 670 nm with a red drop at longer wavelengths. It was concluded that the light absorbed by Photosystem II is responsible for the chemical modification of chloroplasts with p-nitrothiopehnol to causing the specific inhibition of Photosystem II.
在酸性和中性pH条件下，于光照下用对硝基硫酚处理菠菜叶绿体，会导致光系统II活性受到特异性抑制，而在黑暗中进行相同处理则对活性完全没有影响。光系统I的活性在光照和黑暗条件下均未被对硝基硫酚抑制。这种抑制伴随着叶绿体荧光的变化。在室温下观察到，光照下用对硝基硫酚处理会使685nm波段降低，而在液氮温度下，695nm波段与685nm波段的相对高度增加，且695nm波段向更长波长移动。对硝基硫酚对活性和荧光的这些影响的作用光谱在670nm处出现峰值，在更长波长处出现红降。得出的结论是，光系统II吸收的光导致叶绿体被对硝基硫酚化学修饰，从而引起光系统II的特异性抑制。
