小鼠军团菌特异性免疫的评估。

Assessment of legionella-specific immunity in mice.

作者信息

Weber Stefan S, Joller Nicole, Oxenius Annette

机构信息

Institute of Microbiology, ETH Zürich, Zürich, Switzerland.

出版信息

Methods Mol Biol. 2013;954:505-20. doi: 10.1007/978-1-62703-161-5_31.

DOI:10.1007/978-1-62703-161-5_31

PMID:23150417

Abstract

Legionella pneumophila is the causative agent of the potentially fatal Legionnaires' disease in humans. Mice have proved to be valuable model organisms to study the pathogenesis of this intracellular bacterium, as well as immune responses against it. In this chapter we describe a selection of mouse infection protocols to study the innate and adaptive immune responses raised after an infection with Legionella. Included are protocols for systemic and pulmonary infections, surgical collection of organs as well as determination of cell composition, cytokines, and antibody titers therein. Furthermore, we describe an immunohistology protocol to analyze lung tissue sections by fluorescence microscopy.

摘要

嗜肺军团菌是人类潜在致命性军团病的病原体。事实证明，小鼠是研究这种细胞内细菌发病机制以及针对它的免疫反应的有价值的模式生物。在本章中，我们描述了一系列小鼠感染方案，以研究感染军团菌后引发的先天性和适应性免疫反应。其中包括全身和肺部感染的方案、器官的手术采集以及其中细胞组成、细胞因子和抗体滴度的测定。此外，我们还描述了一种免疫组织学方案，用于通过荧光显微镜分析肺组织切片。