Vaughan Catherine, Windle Brad, Deb Sumitra
Department of Integrative Life Sciences, Virginia Commonwealth University, Richmond, VA, USA.
Methods Mol Biol. 2013;962:227-36. doi: 10.1007/978-1-62703-236-0_19.
Mutant p53 may activate target genes through the interaction of transcription factors or through histone modifications. Chromatin immunoprecipitation (ChIP) is a method commonly used to study these types of protein interactions. In order to generate a list of target genes that may be activated through this mechanism, ChIP sequencing may be used. ChIP sequencing involves the mass parallel sequencing of ChIP DNA fragments. We describe a method by which to prepare chromatin immunoprecipitation sequencing libraries and how to analyze sequencing data. In this procedure, prepared libraries have been sent to a core facility. The results have been verified using quantitative PCR.
突变型p53可能通过转录因子的相互作用或通过组蛋白修饰来激活靶基因。染色质免疫沉淀（ChIP）是一种常用于研究这类蛋白质相互作用的方法。为了生成可能通过这种机制被激活的靶基因列表，可使用ChIP测序。ChIP测序涉及对ChIP DNA片段进行大规模平行测序。我们描述了一种制备染色质免疫沉淀测序文库的方法以及如何分析测序数据。在这个过程中，制备好的文库已被送至一个核心设施。结果已通过定量PCR进行了验证。
