Department of Pediatric Infectious Disease, Center for Infectious Diseases and Microbiology Translational Research, University of Minnesota Medical School-Twin Cities, Minneapolis, MN 55455, USA.
J Immunol. 2012 Dec 15;189(12):5503-7. doi: 10.4049/jimmunol.1201413. Epub 2012 Nov 12.
Typhoid fever and nontyphoidal bacteremia caused by Salmonella remain critical human health problems. B cells are required for protective immunity to Salmonella, but the mechanism of protection remains unclear. In this study, we immunized wild-type, B cell-deficient, Ab-deficient, and class-switched Ab-deficient mice with attenuated Salmonella and examined protection against secondary infection. As expected, wild-type mice were protected and B cell-deficient mice succumbed to secondary infection. Interestingly, mice with B cells but lacking secreted Ab or class-switched Ab had little deficiency in resistance to Salmonella infection. The susceptibility of B cell-deficient mice correlated with marked reductions in CD4 T cell IFN-γ production after secondary infection. Taken together, these data suggest that the primary role of B cells in acquired immunity to Salmonella is via the development of protective T cell immunity.
伤寒和非伤寒型沙门氏菌血症仍然是严重的人类健康问题。B 细胞是对沙门氏菌产生保护性免疫所必需的，但保护机制尚不清楚。在这项研究中，我们用减毒沙门氏菌免疫野生型、B 细胞缺陷型、Ab 缺陷型和类别转换 Ab 缺陷型小鼠，并检测对二次感染的保护作用。正如预期的那样，野生型小鼠受到保护，而 B 细胞缺陷型小鼠则死于二次感染。有趣的是，B 细胞存在但缺乏分泌型 Ab 或类别转换 Ab 的小鼠对沙门氏菌感染的抵抗力几乎没有缺陷。B 细胞缺陷型小鼠的易感性与二次感染后 CD4 T 细胞 IFN-γ产生的明显减少相关。综上所述，这些数据表明 B 细胞在获得性对沙门氏菌免疫中的主要作用是通过保护性 T 细胞免疫的发展。
