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对用活化环磷酰胺代谢物处理的L1210小鼠白血病细胞的生物物理研究：DNA链间和DNA-蛋白质交联机制（碱性洗脱和31P-NMR光谱）选择性特性的测定以及联合药物和放射治疗的某些方面。

Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclophosphamide metabolites: determination of selective properties of DNA-interstrand and DNA-protein cross-linking mechanisms (alkaline elution and 31P-NMR-spectroscopy) and some aspects of the combination drug and radiotherapy.

作者信息

Ulmer W

机构信息

FB Radiologie, Abteilung Medizinphysik/Biophysik, St.-Marien-Krankenhaus, Siegen.

出版信息

Strahlenther Onkol. 1990 Feb;166(2):157-63.

PMID:2315845
Abstract

Dose-effect relationships have been studied by using L1210 mouse leukemia cells treated with the activated cyclophosphamide derivatives 4-hydroperoxy-cyclophosphamide and 4-sulfido-cyclophosphamide. The combined treatment of the cells with the drugs and irradiation with X-rays indicates synergistic effects, if the irradiation of the cells is performed four to six hours after the drug treatment. DNA-interstrand and DNA-protein cross-linkings have been determined by an alkaline elution procedure. The yield of cross-linkings has been monitoring by either the 3H-14C method or by a 31P-NMR Fourier spectroscopy analysis of the eluted substrates.

摘要

通过使用经活化的环磷酰胺衍生物4-氢过氧环磷酰胺和4-硫代环磷酰胺处理的L1210小鼠白血病细胞来研究剂量-效应关系。如果在药物处理后四至六小时对细胞进行照射，那么将这些药物与细胞联合处理并进行X射线照射会显示出协同效应。已通过碱性洗脱程序测定了DNA链间交联和DNA-蛋白质交联。交联的产量已通过3H-14C方法或通过对洗脱底物的31P-NMR傅里叶光谱分析进行监测。

相似文献

1
Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclophosphamide metabolites: determination of selective properties of DNA-interstrand and DNA-protein cross-linking mechanisms (alkaline elution and 31P-NMR-spectroscopy) and some aspects of the combination drug and radiotherapy.对用活化环磷酰胺代谢物处理的L1210小鼠白血病细胞的生物物理研究：DNA链间和DNA-蛋白质交联机制（碱性洗脱和31P-NMR光谱）选择性特性的测定以及联合药物和放射治疗的某些方面。
Strahlenther Onkol. 1990 Feb;166(2):157-63.
2
Biophysical aspects of the integrated combination of cytostatic drugs with radiotherapy. Part 1: In vitro investigations on the kinetics of activated isophosphamide metabolites and the determination of selective properties of DNA-interstrand and DNA-protein crosslinking mechanisms using 31P-NMR spectroscopy.
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In vivo DNA cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse L1210 leukemia and normal bone marrow cells.环磷酰胺在体内的DNA交联作用：人慢性淋巴细胞白血病细胞与小鼠L1210白血病细胞及正常骨髓细胞的比较
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Biophysical aspects of the integrated combination of cytostatic drugs with radiotherapy. Part 3: In vivo investigations on murine C3H mammary adenocarcinoma treated with cis-platinum, adriamycin, epirubicin, activated cyclophosphamide, activated isophosphamide and irradiation with 10 MeV electrons--verification of the therapeutic results with 31P-NMR spectroscopy.
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The effect of misonidazole on the cytotoxicity and DNA cross-linking activity of an activated sulfidocyclophosphamide in hypoxic mouse leukemia cells.米索硝唑对缺氧小鼠白血病细胞中活化的硫代环磷酰胺的细胞毒性和DNA交联活性的影响。
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Cross-linking of DNA in L1210 cells and nuclei treated with cyclophosphamide and phosphoramide mustard.用环磷酰胺和磷酰胺氮芥处理的L1210细胞及细胞核中DNA的交联作用。
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