血浆中氨甲蝶呤的高效液相色谱测定法

High-performance liquid chromatographic determination of methotrexate in plasma.

作者信息

Alkaysi H N, Gharaibeh A M, Salem M A

机构信息

Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.

出版信息

Ther Drug Monit. 1990 Mar;12(2):191-4. doi: 10.1097/00007691-199003000-00014.

DOI:10.1097/00007691-199003000-00014

PMID:2315976

Abstract

A procedure for the determination of methotrexate in human plasma is reported. The analysis involved extraction of methotrexate as an ion pair in ethyl acetate. Reconstituted residue was analyzed using reverse phase C-18 column and a mobile phase consisting of acetate buffer (87%), methanol (6.5%), and acetonitrile (6.5%). The methotrexate recovery range was 95-97%. Theophylline was used as internal standard with a recovery of 96%. The intraday coefficient of variation for the assay ranged from 1.8-3.0%, while interday variation coefficient range was 3.5-3.7%. The method is selective, reproducible, and covers a wide range of methotrexate concentrations in patient's plasma.

摘要

本文报道了一种测定人血浆中甲氨蝶呤的方法。该分析过程包括将甲氨蝶呤作为离子对在乙酸乙酯中进行萃取。用反相C-18柱和由醋酸盐缓冲液（87%）、甲醇（6.5%）和乙腈（6.5%）组成的流动相分析复溶后的残渣。甲氨蝶呤的回收率范围为95%-97%。以茶碱作为内标，回收率为96%。该测定方法的日内变异系数范围为1.8%-3.0%，日间变异系数范围为3.5%-3.7%。该方法具有选择性、可重复性，并且能覆盖患者血浆中甲氨蝶呤的广泛浓度范围。