Davis J D, Aarons L, Houston J B
Pharmacy Department, University of Manchester, UK.
J Chromatogr. 1993 Nov 17;621(1):105-9. doi: 10.1016/0378-4347(93)80083-g.
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of theophylline in plasma simultaneously with either ciprofloxacin, enoxacin or norfloxacin has been developed. It involves extraction of plasma with chloroform-isopropanol or dichloromethane-isopropanol prior to chromatography on a Spherisorb ODS2 column. The mobile phase is 15% acetonitrile in a phosphate buffer (pH 3.0) containing tetrabutylammonium hydrogen sulphate (4.4 mM) as an ion-pairing agent. Ultraviolet detection is carried out at 280 nm. Run time is less than 10 min for all three separations. The assays have been used to determine the effect of plasma concentrations of fluoroquinolone on theophylline clearance.
已开发出一种灵敏且具选择性的反相高效液相色谱法，用于同时测定血浆中的茶碱以及环丙沙星、依诺沙星或诺氟沙星。该方法包括在使用Spherisorb ODS2柱进行色谱分析之前，先用氯仿 - 异丙醇或二氯甲烷 - 异丙醇萃取血浆。流动相是在含硫酸氢四丁铵（4.4 mM）作为离子对试剂的磷酸盐缓冲液（pH 3.0）中的15%乙腈。在280 nm处进行紫外检测。所有三种分离的运行时间均少于10分钟。这些测定法已用于确定氟喹诺酮血浆浓度对茶碱清除率的影响。
