Iosefsohn M, Soldin S J, Hicks J M
Department of Laboratory Medicine, Children's National Medical Center, Washington, D.C. 20010.
Ther Drug Monit. 1990 Mar;12(2):201-5. doi: 10.1097/00007691-199003000-00016.
We evaluated the Ames Seralyzer III with a new reagent and drystrip test for assay of digoxin. Assay precision was acceptable in the therapeutic range. Within-run imprecision (coefficient of variation, n = 20) was 7% at 0.9 ng/ml (1.2 nM) and 3.5% at 1.9 ng/ml (2.4 nM); run-to-run imprecision was 7.6% at 0.8 ng/ml (1.0 nM) and 5.7% at 2.1 ng/ml (2.7 nM). The method is very reproducible and is linear between 0.5 and 4.3 ng/ml (0.6-5.5 nM). The assay performed well with patient samples, with Abbott TDx used as the reference procedure. Bilirubin up to 16 mg/dl (273 microM) and hemoglobin up to 11 g/l do not cause interference. Digoxin-like immunoreactive factors cause minimal interference. Some digoxin metabolites such as monodigitoxoside, bis-digitoxoside, and digoxigenin cross-react with the digoxin antibody. Patients on spironolactone have falsely increased digoxin values. The new digoxin assay is easy to perform and uses 30 microliters serum; the result can be reported in 15-20 min.
我们使用一种新试剂和干条试验对Ames Seralyzer III进行了评估，以测定地高辛。在治疗范围内，测定精密度可接受。批内不精密度（变异系数，n = 20）在0.9 ng/ml（1.2 nM）时为7%，在1.9 ng/ml（2.4 nM）时为3.5%；批间不精密度在0.8 ng/ml（1.0 nM）时为7.6%，在2.1 ng/ml（2.7 nM）时为5.7%。该方法具有很高的重现性，在0.5至4.3 ng/ml（0.6 - 5.5 nM）之间呈线性。以雅培TDx作为参考方法，该检测方法对患者样本检测效果良好。胆红素高达16 mg/dl（273 microM）和血红蛋白高达11 g/l时不会产生干扰。地高辛样免疫反应因子产生的干扰最小。一些地高辛代谢物，如单洋地黄毒苷、双洋地黄毒苷和洋地黄毒苷元，会与地高辛抗体发生交叉反应。使用螺内酯的患者地高辛值会假性升高。这种新的地高辛检测方法操作简便，只需30微升血清；结果可在15 - 20分钟内报告。
