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用 Ang2-siRNA 干扰恶性黑素瘤的生长。

Interfering growth of malignant melanoma with Ang2-siRNA.

机构信息

Department of Plastic Surgery, First Affiliated Hospital of Fujian Medical University, Road Chazhong 20, Taijiang, Fuzhou, 350005, China.

出版信息

Mol Biol Rep. 2013 Feb;40(2):1463-71. doi: 10.1007/s11033-012-2189-4. Epub 2012 Nov 16.

DOI:10.1007/s11033-012-2189-4
PMID:23160899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3538013/
Abstract

To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA lent virus, and constructed nude mice model by transplanting malignant melanoma. Ang2-siRNA lent virus inhibited Ang2 mRNA of malignant melanoma in vitro and in vivo, and inhibited malignant melanoma tumor growth at the same time. Ang2-siRNA lent virus can interfere expression levels of Ang2 in malignant melanoma cells, inhibit tumor growth, and silent Ang2 gene expression, which may pave a new way for clinical gene therapy of malignant melanoma.

摘要

为了研究干预治疗对恶性黑色素瘤生长的影响，我们观察了 Ang2 在体外和体内转染恶性黑素瘤细胞的小干扰 RNA（Ang2-siRNA）后表达水平的变化。我们成功构建了 Ang2-siRNA 慢病毒，并通过移植恶性黑素瘤构建了裸鼠模型。Ang2-siRNA 慢病毒抑制了体外和体内恶性黑素瘤的 Ang2mRNA，同时抑制了恶性黑素瘤肿瘤的生长。Ang2-siRNA 慢病毒可以干扰恶性黑素瘤细胞中 Ang2 的表达水平，抑制肿瘤生长，沉默 Ang2 基因表达，这可能为恶性黑素瘤的临床基因治疗开辟新途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/e8b58d062fab/11033_2012_2189_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/71252918a951/11033_2012_2189_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/95933c2246bc/11033_2012_2189_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/f18c9233be58/11033_2012_2189_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/77bbb6fd24f2/11033_2012_2189_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/2052d1aeaeb2/11033_2012_2189_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/325db2726a58/11033_2012_2189_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/108efe89028c/11033_2012_2189_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/ffae9cf15e21/11033_2012_2189_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/e8b58d062fab/11033_2012_2189_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/71252918a951/11033_2012_2189_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/95933c2246bc/11033_2012_2189_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/f18c9233be58/11033_2012_2189_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/77bbb6fd24f2/11033_2012_2189_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/2052d1aeaeb2/11033_2012_2189_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/325db2726a58/11033_2012_2189_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/108efe89028c/11033_2012_2189_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/ffae9cf15e21/11033_2012_2189_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1602/3538013/e8b58d062fab/11033_2012_2189_Fig9_HTML.jpg

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引用本文的文献

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2
Gene therapy for advanced melanoma: selective targeting and therapeutic nucleic acids.晚期黑色素瘤的基因治疗：选择性靶向与治疗性核酸
J Drug Deliv. 2013;2013:897348. doi: 10.1155/2013/897348. Epub 2013 Mar 25.

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Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.血管生成素-2水平与转移性恶性黑色素瘤的疾病进展相关。
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