采用芯片毛细管电泳对 S 型和 R 型内毒素组分进行定量微流控分析。

Quantitative microfluidic analysis of S- and R-type endotoxin components with chip capillary electrophoresis.

机构信息

Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Pécs, Hungary.

出版信息

Electrophoresis. 2012 Nov;33(22):3351-60. doi: 10.1002/elps.201270163.

DOI:10.1002/elps.201270163

Abstract

A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) and rough (R) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole-cell lysates becomes possible with this method. Two groups with three sub-groups in the first group of S-type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S- and R-type Gram-negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS-PAGE combined with silver staining.

摘要

建立了一种新型的快速、灵敏的 ME 方法，用于分析和区分与荧光染料共价标记的光滑（S）和粗糙（R）型细菌内毒素成分。该方法可用于定量分析纯化的脂多糖或来自全细胞裂解物的部分纯化样品。第一组 S 型脂多糖的两个组和三个亚组可以根据电泳图谱进行分类。发现 S 型和 R 型革兰氏阴性菌的内毒素的 LOD 分别为 2.6 ng 和 6.9 ng。该方法可以替代常用的 SDS-PAGE 结合银染法。