Bell J B, Gould R P, Hyatt P J, Tait J F, Tait S A
J Endocrinol. 1979 Dec;83(3):435-47. doi: 10.1677/joe.0.0830435.
The outputs of corticosterone, deoxycorticosterone and androstenedione from dispersed, purified rat adrenal zona reticularis and zona fasciculata cells have been measured by radioimmunoassay. Preferential production of deoxycorticosterone by zona reticularis cells was demonstrated by their higher basal deoxycorticosterone : corticosterone ratio when compared with zona fasciculata cells. Adrencorticotrophin (ACTH) stimulated corticosterone output by all cell pools prepared by unit gravity (1 g) sedimentation, zona fasciculata cells being stimulated 130-fold compared with 20-fold for the zona reticularis cells in relation to their basal corticosterone output. In every cell pool, ACTH stimulated the output corticosterone more than it stimulated the output of deoxycorticosterone. In parallel cell preparations, it was shown that ACTH increased the conversion of tracer amounts of radioactive deoxycorticosterone to corticosterone and decreased the conversion of radioactive corticosterone to 11-dehydrocorticosterone. Adrenocorticotrophin did not increase the conversion of radioactive deoxycorticosterone to total 11-oxygenated steroids (corticosterone + 11-dehydrocorticosterone). It is unlikely therefore that ACTH stimulates 11 beta-hydroxylation. Data indicate that the ratio of deoxycorticosterone to total 11-oxygenated steroids (corticosterone + 11-dehydrocorticosterone) is characteristic for each cell type, and that this ratio will be relatively independent of ACTH stimulation or the amount of pregnenolone substrate available. Basal androstenedione outputs were similar for both types of cell, and ACTH stimulation was very small, being slightly greater for zona fasciculata than for zona reticularis cells. The contribution of the zona reticularis cells to the basal output of any steroid by the cells of the inner two zones of the adrenal cortex of the rat was relatively small (20% for deoxycorticosterone and 10% for corticosterone) and was even less after stimulation by ACTH. Unless a specific stimulus can be found, therefore, a significant role for the zona reticularis cannot yet be established.
采用放射免疫分析法测定了分散纯化的大鼠肾上腺网状带和束状带细胞中皮质酮、脱氧皮质酮和雄烯二酮的分泌量。网状带细胞中脱氧皮质酮的基础分泌量高于束状带细胞，其脱氧皮质酮与皮质酮的基础比值较高，表明网状带细胞优先分泌脱氧皮质酮。促肾上腺皮质激素（ACTH）刺激了所有通过单位重力（1g）沉降制备的细胞池分泌皮质酮，与网状带细胞相比，束状带细胞的皮质酮基础分泌量被刺激了130倍，而网状带细胞仅被刺激了20倍。在每个细胞池中，ACTH对皮质酮分泌的刺激作用大于对脱氧皮质酮分泌的刺激作用。在平行细胞制备实验中，结果表明ACTH增加了微量放射性脱氧皮质酮向皮质酮的转化，并减少了放射性皮质酮向11-脱氢皮质酮的转化。ACTH并没有增加放射性脱氧皮质酮向总11-氧化类固醇（皮质酮+11-脱氢皮质酮）的转化。因此，ACTH不太可能刺激11β-羟化作用。数据表明，脱氧皮质酮与总11-氧化类固醇（皮质酮+11-脱氢皮质酮）的比值是每种细胞类型的特征，并且该比值相对独立于ACTH刺激或可用孕烯醇酮底物的量。两种细胞类型的基础雄烯二酮分泌量相似，ACTH刺激作用非常小，束状带细胞的刺激作用略大于网状带细胞。大鼠肾上腺皮质内两层细胞中，网状带细胞对任何类固醇基础分泌量的贡献相对较小（脱氧皮质酮为20%，皮质酮为10%），ACTH刺激后贡献更小。因此，除非能找到特定的刺激因素，否则尚无法确定网状带的重要作用。
