Yin Xiu-mei, Bai Zhi-chuan, Niu Yun-yun, Luo Hong-mei, Chen Shi-lin
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
Yao Xue Xue Bao. 2012 Aug;47(8):1079-84.
Squalene synthase (SQS) is a key enzyme in plant terpenoid biosynthetic pathway. This study focused on cloning and analysis of Huperzia serrata SQS (HsSQS1) gene. After searching the transcriptome dataset of H serrata, one unique sequence encoding SQS was discovered. The primers were designed according to the transcript sequence of HsSQS1 from the H. serrata transcriptome dataset. The open reading frame of HsSQS1 was cloned using RT-PCR strategy. The bioinformatic analysis of this gene and its corresponding protein were performed. The cDNA (named as HsSQS1) contains a 1263 bp open reading frame and encodes a predicted protein of 420 amino acids. The GenBank accession number for this gene is JQ004938. HsSQS1 contains two transmembrane regions, without signal peptide. The conserved domain of squalene synthase was presented in HsSQS1. HsSQS1 was more abundant in H. serrata root than in leaf and stem. This study cloned and analyzed squalene synthase gene from H. serrata for the first time. The result will provide a foundation for exploring the mechanism ofterpenoid biosynthesis in H. serrata plants.
鲨烯合酶（SQS）是植物萜类生物合成途径中的关键酶。本研究聚焦于蛇足石杉鲨烯合酶（HsSQS1）基因的克隆与分析。在搜索蛇足石杉转录组数据集后，发现了一个编码SQS的独特序列。根据蛇足石杉转录组数据集中HsSQS1的转录序列设计引物。采用RT-PCR策略克隆了HsSQS1的开放阅读框。对该基因及其相应蛋白质进行了生物信息学分析。该cDNA（命名为HsSQS1）包含一个1263 bp的开放阅读框，编码一个预测的420个氨基酸的蛋白质。该基因的GenBank登录号为JQ004938。HsSQS1包含两个跨膜区域，无信号肽。鲨烯合酶的保守结构域存在于HsSQS1中。HsSQS1在蛇足石杉根中的含量比在叶和茎中更丰富。本研究首次克隆并分析了蛇足石杉的鲨烯合酶基因。该结果将为探索蛇足石杉植物萜类生物合成机制提供基础。
