Eriksson B, Arnberg H, Oberg K, Hellman U, Lundqvist G, Wernstedt C, Wilander E
Ludwig Institute for Cancer Research, University Hospital, Uppsala, Sweden.
Acta Endocrinol (Copenh). 1990 Feb;122(2):145-55. doi: 10.1530/acta.0.1220145.
Chromogranins A, B, and C, proteins that are co-stored and co-released with peptides and amines, have been identified in a variety of neuroendocrine tissues, both normal and neoplastic. We examined the secretion of chromogranin A and chromogranin A + B by hormone-producing tumours in patients with endocrine pancreatic tumours, carcinoid tumours, pheochromocytomas, and small cell lung cancer. The radioimmunoassay determining the plasma concentrations of chromogranin A + B showed a greater sensitivity than that determining chromogranin A alone. All patients with endocrine pancreatic tumours, carcinoids, and pheochromocytomas had increased levels of chromogranin A + B, whereas a small number of the patients (5/18 with endocrine pancreatic tumours and 1/3 with pheochromocytomas) had normal levels of chromogranin A. Also in immunocytochemical stainings, our polyclonal antiserum detecting both chromogranin A and B showed a greater sensitivity than other available antisera against chromogranin A, B and C. We have demonstrated that a polyclonal antiserum against a mixture of chromogranin A and B might be a more sensitive marker than chromogranin A alone for diagnosing neuroendocrine tumours. This is not surprising, since both chromogranins are widely distributed in neuroendocrine cells.
嗜铬粒蛋白A、B和C是与肽类和胺类共同储存和共同释放的蛋白质，已在各种正常和肿瘤性神经内分泌组织中被鉴定出来。我们检测了内分泌胰腺肿瘤、类癌、嗜铬细胞瘤和小细胞肺癌患者中激素产生肿瘤分泌嗜铬粒蛋白A和嗜铬粒蛋白A + B的情况。测定血浆嗜铬粒蛋白A + B浓度的放射免疫测定法显示出比单独测定嗜铬粒蛋白A更高的灵敏度。所有内分泌胰腺肿瘤、类癌和嗜铬细胞瘤患者的嗜铬粒蛋白A + B水平均升高，而少数患者（18例内分泌胰腺肿瘤患者中的5例和3例嗜铬细胞瘤患者中的1例）的嗜铬粒蛋白A水平正常。在免疫细胞化学染色中，我们检测嗜铬粒蛋白A和B的多克隆抗血清也比其他针对嗜铬粒蛋白A、B和C的现有抗血清显示出更高的灵敏度。我们已经证明，针对嗜铬粒蛋白A和B混合物的多克隆抗血清可能比单独的嗜铬粒蛋白A是一种更敏感的神经内分泌肿瘤诊断标志物。这并不奇怪，因为两种嗜铬粒蛋白都广泛分布于神经内分泌细胞中。
