采用Cobas 6000开放通道分析法进行丙酮酸的酶法测定。

Enzymatic pyruvate measurement by Cobas 6000 open channel assay.

作者信息

Van den Bossche Dorien, Schiettecatte Johan, Vekens Evilien, De Smet Dieter, Gorus Frans K, Martens Geert A

机构信息

Department of Clinical Chemistry and Radioimmunology, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium.

出版信息

Clin Lab. 2012;58(9-10):1091-5.

PMID:23163130

Abstract

BACKGROUND

Blood pyruvate measurement in conjunction with lactic acid is useful for differentiating pyruvate dehydrogenase deficiencies from primary or secondary disorders of mitochondrial electron transport.

METHODS

We evaluated the analytical performance of pyruvate measurement by an enzymatic open channel assay on a Roche Cobas 6000.

RESULTS

The assay was linear from 0.07 to 0.50 mmol/L pyruvate. Total imprecision ranged from 15.7% to 7.1% at pyruvate levels of 0.08 to 0.31 mmol/L, respectively. Functional sensitivity was 0.07 mmol/L. The assay showed no interference by lipids or bilirubin, whereas haemolysis influenced pyruvate concentrations in a hemoglobin concentration-independent manner. Method comparison with patient samples (n = 41) showed that the Cobas 6000 enzymatic method correlated well (r2 = 0.930) with a similar enzymatic assay on a Cobas Mira platform and showed better accuracy in external control schemes.

CONCLUSIONS

Enzymatic pyruvate measurement by a Cobas 6000 open channel shows satisfactory analytical performance. The assay can be integrated in the automated laboratory workflow and is always ready for use thanks to its on-board reagents.

摘要

背景

结合乳酸进行血液丙酮酸测量，有助于区分丙酮酸脱氢酶缺乏症与线粒体电子传递的原发性或继发性疾病。

方法

我们在罗氏Cobas 6000上通过酶促开放通道测定法评估了丙酮酸测量的分析性能。

结果

该测定法在丙酮酸浓度为0.07至0.50 mmol/L范围内呈线性。在丙酮酸水平分别为0.08至0.31 mmol/L时，总不精密度范围为15.7%至7.1%。功能灵敏度为0.07 mmol/L。该测定法未显示受脂质或胆红素干扰，而溶血以与血红蛋白浓度无关的方式影响丙酮酸浓度。与患者样本（n = 41）的方法比较表明，Cobas 6000酶法与Cobas Mira平台上的类似酶法相关性良好（r2 = 0.930），并且在外部对照方案中显示出更好的准确性。