嗜热高温菌 HB27 漆酶在凯夫拉黑电极上对氧气还原的催化作用。
Catalysis of dioxygen reduction by Thermus thermophilus strain HB27 laccase on ketjen black electrodes.
机构信息
California Institute of Technology, Pasadena, California, USA.
出版信息
J Phys Chem B. 2013 Jan 17;117(2):527-34. doi: 10.1021/jp309759g. Epub 2012 Nov 28.
We present electrochemical analyses of the catalysis of dioxygen reduction by Thermus thermophilus strain HB27 laccase on ketjen black substrates. Our cathodes reliably produce 0.56 mA cm(-2) at 0.0 V vs Ag|AgCl reference at 30 °C in air-saturated buffer, under conditions of nonlimiting O(2) flux. We report the electrochemical activity of this laccase as a function of temperature, pH, time, and the efficiency of its conversion of dioxygen to water. We have measured the surface concentration of electrochemically active species, permitting the extraction of electron transfer rates at the enzyme-electrode interface: 1 s(-1) for this process at zero driving force at 30 °C and a limiting rate of 23 s(-1) at 240 mV overpotential at 50 °C.
我们展示了 Thermus thermophilus 菌株 HB27 漆酶在 Ketjen Black 基底上对氧气还原催化的电化学分析。我们的阴极在 30°C 的空气饱和缓冲液中，在非限制 O2 通量的条件下，可靠地在 0.0 V 对 Ag|AgCl 参考电极产生 0.56 mA cm(-2)的电流。我们报告了这种漆酶的电化学活性作为温度、pH 值、时间和其将氧气转化为水的效率的函数。我们已经测量了电化学活性物质的表面浓度，允许在酶-电极界面提取电子转移速率：在 30°C 零驱动力下，该过程的电子转移速率为 1 s(-1)，在 50°C 时 240 mV 过电势下的极限速率为 23 s(-1)。
Zotero 插件