De Boever J, Kohen F, Leyseele D, Vandekerckhove D
Department of Obstetrics and Gynecology, University Hospital, Gent, Belgium.
J Biolumin Chemilumin. 1990 Jan-Mar;5(1):5-10. doi: 10.1002/bio.1170050103.
We have used isoluminol steroid hormone conjugates in competitive heterogeneous chemiluminescence immunoassays (CIA) for estradiol, progesterone and estriol in body fluids. The assays did not require prior extraction of the steroid hormone with an organic solvent. Polyclonal or monoclonal antibodies, covalently coupled to polyacrylamide microspheres or adsorbed onto the plastic surface of microtritation plates via an intermediate second antibody, were used as solid-phase. The latter solid-phase system allows rapid processing of incubation mixtures. Sensitive and reliable direct CIAs for estradiol in serum, progesterone in serum and in saliva and for estriol in saliva have been developed and their clinical utility has been assessed. Sensitivities ranged from 0.54 to 0.04 nmol/l, and precision (% CV) ranged from 10% to 16%.
我们已将异鲁米诺甾体激素结合物用于体液中雌二醇、孕酮和雌三醇的竞争性非均相化学发光免疫分析（CIA）。该分析无需事先用有机溶剂提取甾体激素。通过中间第二抗体共价偶联到聚丙烯酰胺微球上或吸附到微量滴定板塑料表面的多克隆或单克隆抗体用作固相。后一种固相系统可快速处理孵育混合物。已开发出血清中雌二醇、血清和唾液中孕酮以及唾液中雌三醇的灵敏且可靠的直接CIA，并评估了它们的临床效用。灵敏度范围为0.54至0.04 nmol/l，精密度（%CV）范围为10%至16%。
