Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, China.
Diabetes Res Clin Pract. 2013 Feb;99(2):168-73. doi: 10.1016/j.diabres.2012.05.019. Epub 2012 Nov 17.
To compare the expression of sex hormone binding globulin (SHBG) in normal placental tissues and serum with placental tissues from patients with gestational diabetes mellitus (GDM) and to deduce the mechanism affecting placental SHBG in GDM.
Enzyme-linked immunosorbent assay (ELISA) method were used to detect SHBG levels changes in normal and GDM maternal serum, umbilical cord serum and placental. We measured SHBG mRNA and protein using reverse-transcription polymerase chain reaction, and Western blotting, respectively, in normal and GDM placental tissues.
In maternal serum, compared with the control group, GDM group: glucose, insulin increased, SHBG decreased. In the placenta, compared with the control group, GDM group: SHBG, SHBG mRNA and SHBG protein decreased. There is no correlation between placenta SHBG and maternal serum SHBG respectively in control and GDM group, but in GDM group maternal serum insulin and placenta SHBG are linear correlation.
GDM serum and placental SHBG levels are reduced, hyperinsulinemia may lead to a reduction of SHBG in circulating blood, but also damage the placenta cells which led to placental synthesis and secretion of SHBG decrease.
比较正常胎盘组织和血清与妊娠期糖尿病（GDM）患者胎盘组织中性激素结合球蛋白（SHBG）的表达，推断影响 GDM 胎盘 SHBG 的机制。
采用酶联免疫吸附试验（ELISA）法检测正常和 GDM 母血清、脐血清和胎盘 SHBG 水平的变化。采用逆转录聚合酶链反应（RT-PCR）和 Western blot 法分别检测正常和 GDM 胎盘组织中 SHBG mRNA 和蛋白的表达。
母血清中，与对照组相比，GDM 组：血糖、胰岛素升高，SHBG 降低。胎盘组织中，与对照组相比，GDM 组：SHBG、SHBG mRNA 和 SHBG 蛋白降低。对照组和 GDM 组胎盘 SHBG 与母血清 SHBG 之间均无相关性，但 GDM 组母血清胰岛素与胎盘 SHBG 呈线性相关。
GDM 血清和胎盘 SHBG 水平降低，高胰岛素血症可能导致循环血液中 SHBG 减少，同时也损害胎盘细胞，导致胎盘合成和分泌 SHBG 减少。
