宫颈腺癌的脱氧核糖核酸流式细胞术分析：脱氧核糖核酸倍体和S期分数的预后意义

Deoxyribonucleic acid flow cytometric analysis of cervical adenocarcinoma: prognostic significance of deoxyribonucleic acid ploidy and S-phase fraction.

作者信息

Leminen A, Paavonen J, Vesterinen E, Forss M, Wahlström T, Kulomaa P, Lehtinen M

机构信息

Departments of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.

出版信息

Am J Obstet Gynecol. 1990 Mar;162(3):848-53. doi: 10.1016/0002-9378(90)91022-5.

DOI:10.1016/0002-9378(90)91022-5

PMID:2316596

Abstract

Paraffin-embedded tumor samples from 125 patients with cervical adenocarcinoma were analyzed by deoxyribonucleic acid flow cytometry. Thirty-one percent of the tumors were aneuploid. Triploid deoxyribonucleic acid content predominated (51.3%) and one third of the deoxyribonucleic acid aneuploid tumors were tetraploid, whereas near-diploid deoxyribonucleic acid aneuploidy was seen infrequently. Deoxyribonucleic acid aneuploidy was associated with tumor size, histologic grade, clinical stage, and high S-phase fraction. Deoxyribonucleic acid ploidy and S-phase fraction value were independent prognostic parameters, together with the presence of lymph node metastases and tumor size. In conclusion, our results indicate that flow cytometric deoxyribonucleic acid analysis helps to predict the prognosis and may thus influence the choice of treatment.

摘要

对125例宫颈腺癌患者的石蜡包埋肿瘤样本进行了脱氧核糖核酸流式细胞术分析。31%的肿瘤为非整倍体。三倍体脱氧核糖核酸含量占主导（51.3%），三分之一的脱氧核糖核酸非整倍体肿瘤为四倍体，而近二倍体脱氧核糖核酸非整倍体则很少见。脱氧核糖核酸非整倍体与肿瘤大小、组织学分级、临床分期和高S期分数相关。脱氧核糖核酸倍性和S期分数值是独立的预后参数，与淋巴结转移的存在和肿瘤大小一起。总之，我们的结果表明，流式细胞术脱氧核糖核酸分析有助于预测预后，从而可能影响治疗选择。