Bourne Roger, Bongers Andre, Charles Ned, Power Carl, Sved Paul, Watson Geoffrey
Discipline of Medical Radiation Sciences, Faculty of Health Sciences, University of Sydney, Sydney, New South Wales, Australia.
Magn Reson Med. 2013 Oct;70(4):1160-6. doi: 10.1002/mrm.24549. Epub 2012 Nov 15.
To evaluate the effect of formalin fixation on biexponential modeling of diffusion decay in prostate tissue.
Three whole prostate specimens were imaged unfixed immediately postsurgery, and again after formalin fixation. Diffusion-weighted imaging was performed over an extended range of b-values and a biexponential model fitted to the signal decay curves.
Tissue fixation resulted in a 35%, 20%, and 20% reduction in mean apparent diffusion coefficient of the higher diffusivity fit component for the three organs, respectively, and a 64%, 57%, and 45% reduction in mean apparent diffusion coefficient of the lower diffusivity component. The mean signal fraction of the higher diffusivity component was increased by 23%, 5%, and 1%, respectively. The effect of fixation did not appear to vary according to tissue type or glandular zone.
Formalin fixed tissue appears to provide a stable model for detailed investigation of the microscopic biophysical basis of diffusion phenomena observed in vivo. Diffusivity changes that result from fixation may provide information about the microscopic environments of the biexponential components.
评估福尔马林固定对前列腺组织扩散衰减双指数模型的影响。
三个完整的前列腺标本在手术后立即未固定成像，福尔马林固定后再次成像。在较宽的b值范围内进行扩散加权成像，并对信号衰减曲线拟合双指数模型。
组织固定后，三个器官中高扩散率拟合成分的平均表观扩散系数分别降低了35%、20%和20%，低扩散率成分的平均表观扩散系数分别降低了64%、57%和45%。高扩散率成分的平均信号分数分别增加了23%、5%和1%。固定的影响似乎不随组织类型或腺区而变化。
福尔马林固定的组织似乎为详细研究体内观察到的扩散现象的微观生物物理基础提供了一个稳定的模型。固定导致的扩散率变化可能提供有关双指数成分微观环境的信息。
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