Institute of Bioinformatics, University of Georgia, Athens, Georgia, USA.
PLoS One. 2012;7(11):e48472. doi: 10.1371/journal.pone.0048472. Epub 2012 Nov 15.
The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American hemorrhagic fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80% were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95% bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the host response and pathology of disease.
牙买加果蝠（Artibeus jamaicensis）是热带美洲最常见的蝙蝠之一。它被认为是塔卡里伯病毒的潜在储存宿主，塔卡里伯病毒是一种与南美洲出血热病毒密切相关的沙粒病毒。我们使用 454 和 Illumina 平台对肺、肾和脾组织进行了转录组测序和注释，以开发这种物种作为动物模型。组装了超过 100,000 个连续序列，其中有 25,000 个基因进行了功能注释。在其余未注释的连续序列中，80%位于蝙蝠基因组或转录组内。注释基因涉及广泛的活动，从细胞代谢到通过 ncRNAs 进行基因组调节。相互 BLAST 最佳命中产生了 8,785 个与小鼠、大鼠、牛、马和人类同源的序列。使用来自 2,378 个基因座的序列进行物种树分析，为蝙蝠作为包含马、狗和牛的分支的姐妹群的位置提供了 95%的自举支持。通过在蝙蝠和人类之间估计替代率，确定了 32 个具有正选择证据的基因。我们还鉴定了 466 个与免疫相关的基因，这可能对研究该物种的塔卡里伯病毒感染有用。牙买加果蝠转录组数据集是一个资源，应该为研究蝙蝠进化和生态学提供额外的候选标记，并为分析宿主对疾病的反应和病理学提供工具。
