探索轨道阱分析器用于通过天然质谱法对完整抗体进行表征。
Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry.
机构信息
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands.
出版信息
Angew Chem Int Ed Engl. 2012 Dec 21;51(52):12992-6. doi: 10.1002/anie.201206745. Epub 2012 Nov 22.
PMID:23172610
Abstract
Antibody profiling: native mass spectrometry analysis of intact antibodies can be achieved with improved speed, sensitivity, and mass resolution by using a modified orbitrap instrument. Complex mixtures of monoclonal antibodies can be resolved and their glycan "fingerprints" can be profiled. Noncovalent interactions are maintained, thus allowing antibody-antigen binding to be measured.
摘要
抗体分析
使用改良后的轨道阱仪器，可以提高速度、灵敏度和质量分辨率，实现完整抗体的天然质谱分析。可以解析单克隆抗体的复杂混合物，并分析其聚糖“指纹图谱”。保持非共价相互作用，从而可以测量抗体-抗原结合。
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