Lawrence C W, Christensen R B
Mol Gen Genet. 1979;177(1):31-8. doi: 10.1007/BF00267250.
The UV induced mutation frequency of a given base pair located at different sites within the CYC1 gene of Saccharomyces cerevisiae was found to vary by more than fifty fold, indicating the existence of hotspots and coldspots typical of those found in other organisms. We were unable, however, to find any feature of the nucleotide sequence at or near the sites of mutation that explains this variability. These and other data suggest that hotspots are not located within regions particularly susceptible to the formation of premutational lesions. More probably the variation in mutability depends on differences in the activity of enzymes responsible for producing mutations, though the reasons for these differences are not understood and may depend on factors not directly related to nucleotide sequence.
发现在酿酒酵母CYC1基因内不同位点的特定碱基对的紫外线诱导突变频率变化超过五十倍，这表明存在其他生物体中典型的热点和冷点。然而，我们无法在突变位点或其附近找到任何核苷酸序列特征来解释这种变异性。这些以及其他数据表明，热点并不位于特别容易形成前突变损伤的区域。更有可能的是，突变率的变化取决于负责产生突变的酶的活性差异，尽管这些差异的原因尚不清楚，可能取决于与核苷酸序列没有直接关系的因素。
