采用有培养和无培养方法检测硬蜱体内伯氏疏螺旋体的异质性。
Detection of heterogeneity of Borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods.
机构信息
Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.
出版信息
J Clin Microbiol. 2013 Feb;51(2):615-7. doi: 10.1128/JCM.03009-12. Epub 2012 Nov 21.
Abstract
Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated.
摘要
比较了通过体外培养 (IVC) 和直接 PCR (dPCR) 检测莱姆病螺旋体伯氏疏螺旋体 ospC 基因型异质性的能力。IVC 未能检测到 dPCR 检测到的 ospC 基因型的三分之一。在 IVC 结果中，常见 ospC 基因型被高估，而罕见基因型的出现被低估。
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