Animal Nutrition Institute, Sichuan Agricultural University, Xinkang Road 46#, Ya'an, Sichuan Province 625014, People's Republic of China.
Br J Nutr. 2013 Jun 28;109(12):2253-60. doi: 10.1017/S0007114512004321. Epub 2012 Nov 26.
Oxidative stress is detrimental to animals. Previous studies have indicated that arginine (Arg) may function as a potential substance against oxidative stress. The present study was conducted to explore the potential mechanisms behind the Arg-induced protective effects against oxidative stress in piglets. A total of thirty-six piglets were randomly allocated to six groups with six replicates per group. Piglets were subjected to three dietary treatments (namely two groups per treatment) in week 1 and fed with a basal diet (ArgL) or the basal diet supplemented with 0.8% (ArgM) or 1.6% (ArgH) L-Arg, respectively. On day 8, piglets were injected intraperitoneally either with diquat (10 mg/kg body weight) or sterile saline. The whole trial lasted 11 d. Results showed that dietary Arg supplementation did not affect growth performance in week 1. Oxidative stress significantly decreased the growth performance of piglets (P < 0.05). However, ArgH attenuated the negative effects of oxidative stress on feed intake and significantly increased the total antioxidant capacity in the liver under oxidative stress (P < 0.05). Both ArgM and ArgH enhanced the activities of plasma glutathione peroxidases and superoxide dismutases and decreased the IL-6 and TNF-a mRNA level in the liver under oxidative stress (P < 0.05). The present study not only shows that Arg can function as a potential nutrient to alleviate oxidative stress responses through the enhancement of antioxidant capacity, and inhibition of the expression of inflammatory cytokines, but the results also suggest that alleviation of oxidative stress responses using dietary nutrient components deserves further attention in the future.
氧化应激对动物有害。先前的研究表明，精氨酸（Arg）可能是一种对抗氧化应激的潜在物质。本研究旨在探讨 Arg 诱导的仔猪抗氧化应激保护作用的潜在机制。将 36 头仔猪随机分为 6 组，每组 6 个重复。第 1 周，仔猪接受三种饮食处理（每种处理 2 组），分别饲喂基础日粮（ArgL）或基础日粮添加 0.8%（ArgM）或 1.6%（ArgH）L-Arg。第 8 天，仔猪腹膜内注射百草枯（10 mg/kg 体重）或无菌生理盐水。整个试验持续 11d。结果表明，第 1 周日粮 Arg 补充不影响生长性能。氧化应激显著降低了仔猪的生长性能（P<0.05）。然而，ArgH 减轻了氧化应激对采食量的负面影响，并显著增加了氧化应激下肝脏的总抗氧化能力（P<0.05）。ArgM 和 ArgH 均增强了血浆谷胱甘肽过氧化物酶和超氧化物歧化酶的活性，并降低了氧化应激下肝脏中 IL-6 和 TNF-a mRNA 的水平（P<0.05）。本研究不仅表明 Arg 可作为一种潜在的营养物质，通过增强抗氧化能力和抑制炎症细胞因子的表达来缓解氧化应激反应，而且还表明，未来应进一步关注使用膳食营养成分来缓解氧化应激反应。
