Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales - Universidad de Buenos Aires. Ciudad Universitaria, Pabellón II, Buenos Aires (C1428EHA), Argentina.
Brief Funct Genomics. 2013 Jan;12(1):66-71. doi: 10.1093/bfgp/els053. Epub 2012 Nov 22.
Alternative splicing and post-translational modifications are key events for the generation of proteome diversity in eukaryotes. The study of the molecular mechanisms governing these processes, and every other step of gene expression, has underscored the existing interconnectedness among them. Therefore, molecules that could concertedly regulate different stages from transcription to pre-mRNA processing, translation and even protein activity have called our attention. Serine/arginine-rich proteins, initially identified as splicing regulators, are involved in diverse aspects of gene expression. Although most of the roles exerted by members of this family are related to mRNA biogenesis and metabolism, few recently uncovered ones link these proteins to other regulatory steps along gene expression, particularly the regulation of post-translational modification by conjugation of the small ubiquitin-related modifier. This along with the established link between ubiquitin, transcription and pre-mRNA processing points to a general mechanism of interaction between different cellular machineries, such as ubiquitin/ubiquitin-like conjugation pathways, transcription apparatus and the spliceosome.
可变剪接和翻译后修饰是真核生物产生蛋白质组多样性的关键事件。对这些过程以及基因表达的每一个其他步骤的分子机制的研究强调了它们之间的现有相互关联性。因此，能够协同调节从转录到前体 mRNA 加工、翻译甚至蛋白质活性等不同阶段的分子引起了我们的注意。丝氨酸/精氨酸丰富蛋白最初被鉴定为剪接调节剂，参与基因表达的多个方面。尽管该家族成员发挥的大多数作用与 mRNA 的生物发生和代谢有关，但最近发现的一些作用将这些蛋白与基因表达的其他调节步骤联系起来，特别是通过小泛素相关修饰物的缀合来调节翻译后修饰。这与泛素、转录和前体 mRNA 加工之间的既定联系一起，指向了不同细胞机制之间的一般相互作用机制，如泛素/泛素样缀合途径、转录装置和剪接体。
