Department of Physical Education, University of Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, Las Palmas de Gran Canaria, 35017, Spain.
Int J Sports Med. 2013 Apr;34(4):285-92. doi: 10.1055/s-0032-1312583. Epub 2012 Nov 23.
To determine if the muscle signalling response to a 30 s all-out sprint exercise is modulated by the exercise mode and the endocrine response, 27 healthy volunteers were divided in 2 groups that performed isokinetic (10 men and 5 women) and isoinertial (7 men and 5 women) Wingate tests. Blood samples and vastus lateralis muscle biopsies were taken before, immediately after, 30 and 120 min after the sprints. Groups were comparable in age, height, body weight, percentage of body fat, peak power per kg of lower extremities lean mass (Pmax) and muscle fibre types. However, the isoinertial group achieved a 25% greater mean power (Pmean). Sprint exercise elicited marked increases in the musculus vastus lateralis AMPKα, ACCβ, STAT3, STAT5 and ERK1/2 phosphorylation (all P<0.05). The AMPKα, STAT3, and ERK1/2 phosphorylation responses were more marked after the isoinertial than isokinetic test (interaction: P<0.01). The differences in muscle signalling could not be accounted for by differences in Pmax, although Pmean could explain part of the difference in AMPKα phosphorylation. The leptin, insulin, glucose, GH, IL-6, and lactate response were similar in both groups. In conclusion, the muscle signalling response to sprint exercise differs between isoinertial and isokinetic sprints.
为了确定 30 秒全力冲刺运动的肌肉信号反应是否受到运动方式和内分泌反应的调节，27 名健康志愿者被分为两组，分别进行等动（10 名男性和 5 名女性）和等张（7 名男性和 5 名女性）Wingate 测试。在冲刺前、冲刺后即刻、30 分钟和 120 分钟采集血样和股外侧肌活检。两组在年龄、身高、体重、体脂百分比、下肢去脂体重的峰值功率（Pmax）和肌纤维类型方面具有可比性。然而，等张组的平均功率（Pmean）增加了 25%。冲刺运动引起股外侧肌 AMPKα、ACCβ、STAT3、STAT5 和 ERK1/2 磷酸化显著增加（均 P<0.05）。与等动测试相比，等张测试后 AMPKα、STAT3 和 ERK1/2 磷酸化反应更为明显（交互作用：P<0.01）。虽然 Pmean 可以解释 AMPKα 磷酸化差异的一部分，但肌肉信号的差异不能用 Pmax 的差异来解释。两组的瘦素、胰岛素、血糖、GH、IL-6 和乳酸反应相似。总之，等张和等动冲刺运动的肌肉信号反应不同。
