Department of Physical Education, Campus Universitario de Tafira, University of Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Spain.
Eur J Appl Physiol. 2012 May;112(5):1917-27. doi: 10.1007/s00421-011-2164-0. Epub 2011 Sep 18.
To determine if there is a sex dimorphism in the skeletal muscle signaling response to sprint exercise, 17 men and ten women performed a 30-s Wingate test. Muscle biopsies were taken before, immediately after the exercise and at 30 and 120 min during the recovery period. Thr(172)-AMPKα, Ser(221)-ACCβ, Thy(705)-STAT3, Thr(202)/Thy(204)-ERK1/2 and Thr(180)/Thy(182)-p38MAPK phosphorylation responses to sprint exercise were not statistically different between men and women. AMPKα phosphorylation was enhanced fourfold 30 min after the sprint exercise in males and females (P < 0.01). ACCβ phosphorylation was enhanced by about threefold just after the sprint test exercise and 30 min into the recovery period in males and females (P < 0.01). STAT3 phosphorylation was increased 2 h after the Wingate test compared to the value observed right after the end of the exercise (P < 0.05), and 30 min after the Wingate test there was a 2.5-fold increase in ERK1/2 phosphorylation, compared to both the pre-exercise and to the value observed right after the Wingate test (both, P < 0.05). In conclusion, the skeletal muscle signaling response to a single bout of sprint exercise mediated by AMPK, ACC, STAT3, ERK and p38MAPK is not statistically different between men and women. Marked increases in AMPKα, ACCβ, STAT3 and ERK phosphorylation were observed after a single 30-s all-out sprint (Wingate test) in the vastus lateralis.
为了确定冲刺运动对骨骼肌信号的反应是否存在性别二态性，17 名男性和 10 名女性进行了 30 秒的 Wingate 测试。在运动前、运动后立即以及恢复期间的 30 和 120 分钟时，采集了肌肉活检。男性和女性的 Thr(172)-AMPKα、Ser(221)-ACCβ、Thy(705)-STAT3、Thr(202)/Thr(204)-ERK1/2 和 Thr(180)/Thy(182)-p38MAPK 磷酸化反应在统计学上没有性别差异。男性和女性的 AMPKα 磷酸化在冲刺运动后 30 分钟增强了四倍（P < 0.01）。ACCβ 磷酸化在冲刺测试后立即和恢复期间的 30 分钟增强了约三倍（P < 0.01）。STAT3 磷酸化在 Wingate 测试后 2 小时比运动结束后立即观察到的数值增加（P < 0.05），并且在 Wingate 测试后 30 分钟，与运动前和 Wingate 测试后立即观察到的数值相比，ERK1/2 磷酸化增加了 2.5 倍（均，P < 0.05）。总之，单次冲刺运动介导的骨骼肌信号反应中 AMPK、ACC、STAT3、ERK 和 p38MAPK 在男性和女性之间没有统计学差异。在单次 30 秒全力冲刺（Wingate 测试）后，股外侧肌中观察到 AMPKα、ACCβ、STAT3 和 ERK 磷酸化明显增加。
