Food Research and Development Centre (FRDC), Agriculture and Agri-Food Canada, St Hyacinthe, Quebec, Canada.
J Dairy Res. 2013 Feb;80(1):58-63. doi: 10.1017/S0022029912000660. Epub 2012 Nov 27.
Caseinomacropeptide (CMP), a 7-kDa phosphoglycopolypeptide fragment released from κ-casein during milk renneting, is heterogeneous with respect to post-translational glycosylation. Several studies have reported that CMP has growth-promoting activity on lactic acid bacteria belonging to the genera Bifidobacterium. The aim of this study was to evaluate the effect of glycosylation and sequence variations between bovine and caprine CMP on the growth of two probiotics: Lactobacillus rhamnosus RW-9595-M and Bifidobacterium thermophilum RBL67. The growth-promoting activities of CMP (mixture of glycosylated (gCMP) and non-glycosylated (aCMP) fractions), aCMP and gCMP were measured in a basal minimal culture medium using turbidimetric microplate assay at 37 °C. Supplementation of the culture media at 2 mg/ml significantly improved maximum growth by 1.5 to 1.8 times depending on the strain, the additive (CMP, aCMP, gCMP), and the bovine or caprine origin (P < 0.05). CMP preparations also decreased the time needed to reach the inflexion point of the growth curve and increase the cell density at that time (P < 0.05). The effects of CMP preparations were dose dependent and significantly superior to the effect of bovine β-lactoglobulin added to the culture media. As gCMP and aCMP were as efficient as bovine and caprine CMP (P > 0.1), it was concluded that the presence of oligosaccharides linked to CMP was not essential for growth-promoting activity of CMP.
-caseinomacropeptide (CMP),κ-酪蛋白在凝乳酶作用下释放的 7kDa 磷酸糖肽片段,在翻译后糖基化方面具有不均一性。有几项研究报道 CMP 对双歧杆菌属和乳杆菌属的乳酸菌具有促生长活性。本研究旨在评估牛源和羊源 CMP 之间在糖基化和序列变化方面的差异对两种益生菌(鼠李糖乳杆菌 RW-9595-M 和嗜热双歧杆菌 RBL67)生长的影响。使用浊度微板测定法在 37°C 的基础最小培养基中测量 CMP(糖基化(gCMP)和非糖基化(aCMP)级分的混合物)、aCMP 和 gCMP 的促生长活性。在培养基中添加 2mg/ml 的添加剂(CMP、aCMP、gCMP)显著提高了最大生长速度,提高了 1.5 至 1.8 倍,这取决于菌株、添加剂(CMP、aCMP、gCMP)和牛源或羊源(P<0.05)。CMP 制剂还缩短了达到生长曲线拐点所需的时间,并增加了此时的细胞密度(P<0.05)。CMP 制剂的作用呈剂量依赖性,明显优于向培养基中添加牛β-乳球蛋白的作用。由于 gCMP 和 aCMP 与牛源和羊源 CMP 一样有效(P>0.1),因此可以得出结论,CMP 上连接的寡糖的存在对 CMP 的促生长活性不是必需的。