Leoncini G, Maresca M, Buzzi E, Piana A, Armani U
Istituto Policattedra di Chirnica Biologica, Università di Genova, Italy.
Eur J Haematol. 1990 Feb;44(2):116-20. doi: 10.1111/j.1600-0609.1990.tb00361.x.
The adenine nucleotides present in distinct cellular compartments of platelets of 27 patients affected with essential thrombocythemia have been measured. In order to quantify granule-bound nucleotides and adenylic cytoplasmic pool, platelets have been stimulated with thrombin or treated with increasing digitonin concentrations, respectively. Among patients, we have identified two groups: 12 patients (Group 1) had normal platelet level of ATP and ADP both in dense granules as well as in cytoplasmic pool. The other patients (Group 2) had granule ATP and ADP significantly lower and ATP/ADP ratio significantly higher than controls. In these patients an increase in hypoxanthine level, derived from metabolic ATP degradation occurring during stimulation, was observed. In addition, in the latter patients an increased resistance of plasma membrane to digitonin was shown, suggesting that membrane fluidity should be reduced owing to a modified cholesterol/phospholipid ratio.
对27例原发性血小板增多症患者血小板不同细胞区室中的腺嘌呤核苷酸进行了测定。为了量化颗粒结合核苷酸和腺苷酸细胞质池，分别用凝血酶刺激血小板或用递增浓度的洋地黄皂苷处理血小板。在患者中，我们确定了两组：12例患者（第1组）致密颗粒和细胞质池中ATP和ADP的血小板水平正常。其他患者（第2组）的颗粒ATP和ADP明显低于对照组，ATP/ADP比值明显高于对照组。在这些患者中，观察到刺激期间代谢ATP降解产生的次黄嘌呤水平升高。此外，在后者患者中，显示出质膜对洋地黄皂苷的抗性增加，这表明由于胆固醇/磷脂比例的改变，膜流动性应降低。
