Wion D, Mac Grogan D, Houlgatte R, Brachet P
INSERM U 298, Centre Hospitalier Régional et Universitaire, Angers, France.
FEBS Lett. 1990 Mar 12;262(1):42-4. doi: 10.1016/0014-5793(90)80149-d.
The rise of the NGF mRNA pool which takes place following exposure of L-929 fibroblasts to serum was prevented in the presence of 5 microM K-252a, a compound which inhibits several species of protein kinase activities. To characterize further this phenomenon, L-929 cells growing in a serum-free medium were exposed to cyclic nucleotide analogs, to a divalent cation ionophore or to the phorbol ester PMA. Only this latter compound induced an enhancement of the NGF mRNA pool, suggesting an involvement of protein kinase C in the upregulation of the NGF transcripts. The effects of PMA or serum also require a synthesis of protein since the level of NGF transcripts remained stable in the presence of cycloheximide.
将L - 929成纤维细胞暴露于血清后发生的NGF mRNA池的增加，在存在5 microM K - 252a（一种抑制多种蛋白激酶活性的化合物）的情况下受到了抑制。为了进一步表征这一现象，将在无血清培养基中生长的L - 929细胞暴露于环核苷酸类似物、二价阳离子离子载体或佛波酯PMA。只有后一种化合物诱导了NGF mRNA池的增加，这表明蛋白激酶C参与了NGF转录本的上调。PMA或血清的作用也需要蛋白质合成，因为在存在环己酰亚胺的情况下，NGF转录本的水平保持稳定。
