Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, Catedrático Rodrigo Uría s/n, 33071 Oviedo, Spain.
J Hazard Mater. 2013 Jan 15;244-245:555-62. doi: 10.1016/j.jhazmat.2012.10.044. Epub 2012 Oct 30.
Two clones of Dittrichia viscosa (L.) Greuter from contrasting populations, DV-A (metallicolous) and DV-W (non-metallicolous), were studied to compare Cd accumulation and tolerance. After 10 days of hydroponic culture with 0, 5, 10, and 15 mg Cd L(-1), metal accumulation and plant growth were measured as well as other stress markers such as decrease in the content of photosynthetic pigments, lipid peroxidation, phenols, H(2)O(2), and free proline. We also analyzed the activity of the antioxidant enzymes guaiacol and ascorbate peroxidases, catalase, superoxide dismutase, and glutathione reductase as well as their isoform patterns. Our results confirmed a high Cd tolerance and accumulation in both clones of D. viscosa, which suggests that these traits are constitutive in this species. However, when the Cd concentration in solution exceeded 10 mg Cd L(-1), DV-A was more tolerant than DV-W. The physiological mechanisms involved in Cd tolerance also differed between them, although phenols and guaiacol peroxidase played an important role in both clones. The effective Cd detoxification of DV-A consisted mainly in a promoted ascorbate peroxidase activity and better efficiency of catalase and glutathione reductase enzymes.
从两个具有对比性生境的二歧飘拂草（DV-A，金属富养型；DV-W，非金属富养型）克隆体中选择了两个克隆体进行 Cd 积累和耐性的比较研究。在含有 0、5、10 和 15 mg Cd L(-1)的水培条件下培养 10 天后，我们测量了金属积累和植物生长情况以及其他应激指标，如光合色素含量降低、脂质过氧化、酚类、H(2)O(2)和游离脯氨酸的含量。我们还分析了抗氧化酶（愈创木酚过氧化物酶和抗坏血酸过氧化物酶）、过氧化氢酶、超氧化物歧化酶和谷胱甘肽还原酶的活性及其同工酶模式。我们的研究结果证实了二歧飘拂草的两个克隆体具有高 Cd 耐性和积累能力，这表明这些特性是该物种的固有特性。然而，当溶液中的 Cd 浓度超过 10 mg Cd L(-1)时，DV-A 比 DV-W 更具耐性。尽管酚类和愈创木酚过氧化物酶在两个克隆体中都发挥了重要作用，但它们在 Cd 耐性中涉及的生理机制也有所不同。DV-A 的有效 Cd 解毒主要依赖于增强的抗坏血酸过氧化物酶活性和更高效率的过氧化氢酶和谷胱甘肽还原酶。
