Ajiboye Taofeek Olakunle, Komolafe Yesirat Omonike, Bukoye Oloyede Hussein Oyelola, Yakubu Musa Toyin, Adeoye Moriam Dasola, Abdulsalami Ibrahim Olasegun, Oladiji Adenike Temidayo, Akanji Musbau Adewumi
Antioxidants, Free Radicals and Toxicology Research Laboratory, Biochemistry and Nutrition Unit, Department of Chemical Sciences, Fountain University, Osogbo, Nigeria.
J Basic Clin Physiol Pharmacol. 2013;24(1):41-9. doi: 10.1515/jbcpp-2012-0031.
This study investigates the protective role of polyphenolic-rich extract from Sorghum bicolor against diethylnitrosamine (DEN)-induced redox imbalance in rat microsomes.
Reactive oxygen species (ROS) scavenging potentials of the polyphenolic extract from S. bicolor (0.2-1.0 mg/mL) was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical, and ferric ion reducing system. The detoxification of ROS was evaluated in DEN-induced redox imbalance in rat microsomes.
Sorghum bicolor polyphenolic extract at 1.0 mg/mL scavenged the DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical at 75%, 76%, 79%, and 81%, respectively; it also reduced ferric ion significantly. The polyphenolic extract significantly (p<0.05) attenuated DEN-mediated decrease in the activities of ROS detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase and reductase, and glucose-6-phosphate dehydrogenase). The concentrations of malondialdehyde, conjugated dienes, lipid hydroperoxide, protein carbonyl, and percentage DNA fragmentation in DEN-treated microsomes were significantly reduced by the polyphenolic extract.
The results of the present study indicated that S. bicolor polyphenolic extract possessed in vitro antioxidant activity and protected microsomes from DEN-mediated oxidative stress by scavenging free radicals and ROS scavenger and inducer of ROS detoxifying enzymes.
本研究调查了高粱富含多酚的提取物对二乙基亚硝胺（DEN）诱导的大鼠微粒体氧化还原失衡的保护作用。
使用2,2-二苯基-1-苦基肼（DPPH）自由基、超氧离子、过氧化氢、羟基自由基和铁离子还原系统，研究了高粱（0.2 - 1.0 mg/mL）多酚提取物的活性氧（ROS）清除潜力。在DEN诱导的大鼠微粒体氧化还原失衡中评估ROS的解毒作用。
1.0 mg/mL的高粱多酚提取物分别清除DPPH、超氧离子、过氧化氢和羟基自由基的比例为75%、76%、79%和81%；它还能显著还原铁离子。多酚提取物显著（p<0.05）减轻了DEN介导的ROS解毒酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和还原酶以及葡萄糖-6-磷酸脱氢酶）活性的降低。多酚提取物显著降低了DEN处理的微粒体中丙二醛、共轭二烯、脂质氢过氧化物、蛋白质羰基的浓度以及DNA片段化百分比。
本研究结果表明，高粱多酚提取物具有体外抗氧化活性，通过清除自由基以及作为ROS清除剂和ROS解毒酶诱导剂，保护微粒体免受DEN介导的氧化应激。
Zotero 插件