Department of Organic and Biological Chemistry, University of Messina, Viale F. Stagno d'Alcontres 31, 98166, Messina, Italy.
Cell Biochem Biophys. 2013 Jun;66(2):297-307. doi: 10.1007/s12013-012-9485-4.
The bioprotective action of the disaccharide trehalose has been studied against the well-known denaturating agent, guanidine hydrochloride. The results indicated a direct influence of trehalose on both enzymatic activity and conformational changes of lysozyme, as shown by the decrease of the inactivation rate constant of about 1.48-fold and the loss of α-helix structure of lysozyme. In addition, ESI-MS hydrogen-deuterium (H/D) exchange experiments allowed us to correlate the structural and dynamic features of the protein in the presence of the two additives, highlighting as trehalose remarkably influenced this exchange by decreasing local protein environment changes and solvent accessibility to the amide peptide backbone, as further evidenced by circular dichroism and (1)H NMR measurements.
二糖海藻糖的生物保护作用已针对著名的变性剂盐酸胍进行了研究。结果表明，海藻糖直接影响溶菌酶的酶活性和构象变化，其失活速率常数降低约 1.48 倍，溶菌酶的α-螺旋结构丢失。此外，ESI-MS 氢氘（H/D）交换实验使我们能够在存在两种添加剂的情况下关联蛋白质的结构和动态特征，结果表明海藻糖通过降低局部蛋白质环境变化和对酰胺肽骨架的溶剂可及性显著影响这种交换，圆二色性和（1）H NMR 测量进一步证明了这一点。
