Yang Fei, Cao Meng, Jin Yi, Yang Xiushan, Tian Shen
College of Life Science, Capital Normal University, Beijing 100048, China.
Sheng Wu Gong Cheng Xue Bao. 2012 Aug;28(8):901-11.
Saccharomyces cerevisiae is useful as a host for genetic engineering, since it allows the folding and glycosylation of expressed heterologous eukaryotic proteins and can be subjected to many genetic manipulations. Recent advancements in the yeast cell surface engineering developed strategies to genetically immobilize amylolytic, cellulolytic and xylanolytic enzymes on yeast cell surface for the production of fuel ethanol from biomass. We reviewed the basic principle and progress of S. cerevisiae cell-surface engineering and gave an insight into the recent technological developments in the production of bioethanol using surface engineered yeast.
酿酒酵母作为基因工程的宿主很有用,因为它能使表达的异源真核蛋白进行折叠和糖基化,并且可以进行多种基因操作。酵母细胞表面工程的最新进展开发了一些策略,可将淀粉分解酶、纤维素分解酶和木聚糖分解酶基因固定在酵母细胞表面,用于从生物质生产燃料乙醇。我们综述了酿酒酵母细胞表面工程的基本原理和进展,并深入探讨了使用表面工程酵母生产生物乙醇的最新技术发展。