a Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
Bioengineered. 2017 Mar 4;8(2):115-119. doi: 10.1080/21655979.2016.1212135. Epub 2016 Jul 26.
The cell surface serves as a functional interface between the inside and the outside of the cell. Within the past 20 y the ability of yeast (Saccharomyces cerevisiae) to display heterologous proteins on the cell surface has been demonstrated. Furthermore, S. cerevisiae has been both developed and applied in expression of various proteins on the cell surface. Using this novel and useful strategy, proteins and peptides of various kinds can be displayed on the yeast cell surface by fusing the protein of interest with the glycosylphosphatidylinositol (GPI)-anchoring system. Consolidated bioprocessing (CBP) using S. cerevisiae represents a promising technology for bioethanol production. However, further work is needed to improve the fermentation performance. There is some excellent previous research regarding construction of yeast biocatalyst using the surface display system to decrease cost, increase efficiency of ethanol production and directly utilize starch or biomass for fuel production. In this commentary, we reviewed the yeast surface display system and highlighted recent work. Additionally, the strategy for decrease of phytate phosphate content in dried distillers grains with solubles (DDGS) by display of phytase on the yeast cell surface is discussed.
细胞表面是细胞内外功能的界面。在过去的 20 年中，酵母（酿酒酵母）能够在细胞表面展示异源蛋白的能力已经得到证实。此外，酿酒酵母已被开发并应用于细胞表面各种蛋白质的表达。利用这种新颖有用的策略，可以通过将感兴趣的蛋白质与糖基磷脂酰肌醇（GPI）锚定系统融合，将各种蛋白质和肽类物质展示在酵母细胞表面。利用酿酒酵母进行综合生物加工（CBP）代表了生产生物乙醇的一项很有前途的技术。然而，需要进一步的工作来提高发酵性能。之前有一些关于使用表面展示系统构建酵母生物催化剂的优秀研究，旨在降低成本、提高乙醇生产效率，并直接利用淀粉或生物质生产燃料。在本评论中，我们回顾了酵母表面展示系统，并强调了最近的工作。此外，还讨论了通过在酵母细胞表面展示植酸酶降低干燥酒糟中植酸磷含量的策略。
