Zang Hong, Zhao Jing-Min, Ji Dong, Sun Yan-Ling, Zhou Guang-De, Zhao Yu-Lai, Chen Guo-Feng
302 Hospital of PLA, Beijing 100039, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2012 Aug;26(4):285-7.
To explore whether the cellular apoptosis susceptibility (CAS) protein could serve as a pathologic marker for HCC diagnosis and the roles of CAS expression in HBV infection associated HCC.
The expression of CAS protein in HCC and its paracarcinoma tissues, non-tumor liver cirrhosis and hepatitis tissues were detected by immunohistochemistry. Meanwhile, HBsAg, HBcAg and HBV DNA in HCC tissues with HBV infection were examined by immunohistochemistry and in situ hybridization respectively.
The expression of CAS protein was significantly higher in HCC than in its paracarcinomas tissues (P < 0.01), and higher in paracarcinomas tissues than in non-tumor liver cirrhosis and hepatitis tissues (P < 0.01). Poorly differentiated tumors immunochemically stained stronger than moderately or well differentiated (P < 0.01). CAS protein expression was significantly higher in HBV-infected HCC tissues than that of in non-HBV infection (P < 0.01). Meanwhile, in HBV-infected HCC tissues, the staining intensity score of CAS protein in HBV DNA positive HCC tissues was significantly higher than HBV DNA negative tissues (P < 0.05).
Higher expression of CAS protein is found in HCC tissues,and the intensity of CAS protein expression is related closely to tumor differentiation. We suggested that CAS protein might serve as a marker for HCC diagnosis and differentiation estimation, and deduced that CAS might play an important role in the initiation of HBV infection associated HCC through upregulating expression of CAS.
探讨细胞凋亡易感性（CAS）蛋白是否可作为肝癌诊断的病理标志物以及CAS表达在乙型肝炎病毒（HBV）感染相关肝癌中的作用。
采用免疫组织化学法检测CAS蛋白在肝癌及其癌旁组织、非肿瘤性肝硬化和肝炎组织中的表达。同时，分别采用免疫组织化学和原位杂交法检测HBV感染的肝癌组织中的HBsAg、HBcAg和HBV DNA。
CAS蛋白在肝癌中的表达显著高于其癌旁组织（P<0.01），在癌旁组织中的表达高于非肿瘤性肝硬化和肝炎组织（P<0.01）。低分化肿瘤的免疫化学染色强于中分化或高分化肿瘤（P<0.01）。HBV感染的肝癌组织中CAS蛋白表达显著高于非HBV感染的肝癌组织（P<0.01）。同时，在HBV感染的肝癌组织中，HBV DNA阳性的肝癌组织中CAS蛋白的染色强度评分显著高于HBV DNA阴性的组织（P<0.05）。
肝癌组织中CAS蛋白表达较高，且CAS蛋白表达强度与肿瘤分化密切相关。我们认为CAS蛋白可能作为肝癌诊断和分化评估的标志物，并推断CAS可能通过上调表达在HBV感染相关肝癌的发生中起重要作用。
