Facultad de Ingeniería Química, Benemérita Universidad Autónoma de Puebla, Puebla, México.
Lett Appl Microbiol. 2013 Feb;56(2):142-8. doi: 10.1111/lam.12027. Epub 2012 Dec 20.
Multiple-pass ultrahigh pressure homogenization (UHPH) was used for reducing microbial population of both indigenous spoilage microflora in whole raw milk and a baroresistant pathogen (Staphylococcus aureus) inoculated in whole sterile milk to define pasteurization-like processing conditions. Response surface methodology was followed and multiple response optimization of UHPH operating pressure (OP) (100, 175, 250 MPa) and number of passes (N) (1-5) was conducted through overlaid contour plot analysis. Increasing OP and N had a significant effect (P < 0·05) on microbial reduction of both spoilage microflora and Staph. aureus in milk. Optimized UHPH processes (five 202-MPa passes; four 232-MPa passes) defined a region where a 5-log(10) reduction of total bacterial count of milk and a baroresistant pathogen are attainable, as a requisite parameter for establishing an alternative method of pasteurization. Multiple-pass UHPH optimized conditions might help in producing safe milk without the detrimental effects associated with thermal pasteurization.
多道超高压匀质（UHPH）用于减少生牛乳中原生腐败微生物菌群和接种于无菌生牛乳中的耐压病原体（金黄色葡萄球菌）的种群数量，以定义类似于巴氏杀菌的加工条件。采用响应面法，通过叠加等高线图分析对 UHPH 操作压力（OP）（100、175、250 MPa）和通过数（N）（1-5）进行多响应优化。增加 OP 和 N 对牛奶中腐败微生物菌群和金黄色葡萄球菌的微生物减少有显著影响（P<0.05）。优化的 UHPH 工艺（五次 202 MPa 道次；四次 232 MPa 道次）定义了一个区域，在该区域可以实现牛奶总细菌计数和耐压病原体减少 5 个对数（10），这是建立巴氏杀菌替代方法的必要参数。多道 UHPH 优化条件可能有助于生产安全的牛奶，而不会产生与热巴氏杀菌相关的不利影响。
