National Food Institute, Technical University of Denmark, Lyngby, Denmark.
J Agric Food Chem. 2012 Dec 19;60(50):12388-96. doi: 10.1021/jf303560f. Epub 2012 Dec 7.
The objective of this study was to investigate the oxidative stability of dispersions prepared from different levels of purified marine phospholipid (PL) obtained by acetone precipitation, with particular focus on the interaction between α-tocopherol and PL in dispersions. This also included the investigation of nonenzymatic browning in purified marine PL dispersions. Dispersions were prepared by high-pressure homogenizer. The oxidative and hydrolytic stabilities of dispersions were investigated by determination of hydroperoxides, secondary volatile oxidation products, and free fatty acids, respectively, during 32 days of storage at 2 °C. Nonenzymatic browning was investigated through measurement of Strecker aldehydes, color changes, and pyrrole content. Dispersions containing α-tocopherol or higher levels of purified marine PL showed a lower increment of volatiles after 32 days storage. The results suggested that tocopherol is an efficient antioxidant in PL dispersions or that the presence of α-tocopherol and pyrroles may be the main reason for the high oxidative stability of purified marine PL dispersions.
本研究旨在探讨不同纯度海洋磷脂（PL）水平的分散体系的氧化稳定性，特别关注分散体系中α-生育酚与 PL 之间的相互作用。同时，本研究还考察了纯化海洋 PL 分散体系中的非酶褐变现象。采用高压均质机制备分散体系。通过测定 32 天内 2℃储存期间的氢过氧化物、二次挥发性氧化产物和游离脂肪酸，分别考察了分散体系的氧化和水解稳定性。通过测定斯特克醛、颜色变化和吡咯含量来研究非酶褐变。含有 α-生育酚或更高纯度海洋 PL 的分散体系在 32 天后储存时挥发性物质的增量较低。结果表明，生育酚是 PL 分散体系中一种有效的抗氧化剂，或者 α-生育酚和吡咯的存在可能是纯化海洋 PL 分散体系具有高氧化稳定性的主要原因。
