Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2013 Jan;128:107-12. doi: 10.1016/j.biortech.2012.09.123. Epub 2012 Oct 12.
To characterize lipid content of microalgal cells rapidly and accurately, the gravimetric determination, FT-IR and Nile Red (NR) staining were investigated on six typical eukaryotic and prokaryotic algae species. FT-IR and Nile Red were relative quantification methods and a standard curve was required in contrast to the gravimetric method. The FT-IR method determined the lipid, carbohydrate and protein contents simultaneously assuming that the algal cells only consisted of those three components. The Nile Red method was a relatively rapid method for neutral lipid content characterization by spectrofluorometry and could locate lipid body of the algal cell by fluorescence microscopy. According to sample sources and processing purposes, the gravimetric determination was preferable for large-scale cultivation with low-frequency monitoring, while FT-IR and Nile Red were suitable for general laboratory cultivation with medium-frequency monitoring, in particularly Nile Red was appropriate for small samples when high-frequency screening was required.
为了快速准确地描述微藻细胞的脂质含量，研究了 6 种典型的真核和原核藻类的重量法、FT-IR 和尼罗红（NR）染色法。FT-IR 和尼罗红是相对定量方法，与重量法相比需要标准曲线。FT-IR 法同时测定脂质、碳水化合物和蛋白质含量，假设藻类细胞仅由这三种成分组成。尼罗红法是一种通过荧光光谱法快速测定中性脂质含量的方法，通过荧光显微镜可以定位藻类细胞的脂体。根据样品来源和处理目的，重量法适用于低频监测的大规模培养，而 FT-IR 和尼罗红法适用于中频率监测的一般实验室培养，特别是当需要高频筛选时，尼罗红法适用于小样本。
