Akamatsu Wado
Keio University, School of Medicine.
Rinsho Shinkeigaku. 2012;52(11):937-8. doi: 10.5692/clinicalneurol.52.937.
The rapid and efficient induction of neural stem cells (NSCs) from pluripotent stem cells is required for the research of patient-specific iPS cells and regenerative medicine to induce their own neural cells. Here, we induced NSCs from human pluripotent stem cells within 2 weeks and these clonal NSCs were expanded efficiently by their self-renewal ability. Further, we directly induced NSCs from both mouse and human fibroblasts using four reprogramming factors (Oct4, Sox2, Klf4, and cMyc) without the clonal isolation of induced pluripotent stem cells (iPSCs). Since these NSCs rapidly developed into mature gliogenic neural stem cells, we were able to purify these rapidly differentiating NSCs without contamination of differentiation-resistant pluripotent cells. These methods will facilitate high throughput screening of phonotypes appeared in neural cells induced from the somatic cells derived from sporadic neurodegenerative diseases.
从多能干细胞快速高效地诱导神经干细胞（NSCs）是患者特异性诱导多能干细胞（iPS细胞）研究和再生医学中诱导自身神经细胞所必需的。在此，我们在2周内从人多能干细胞诱导出NSCs，并且这些克隆的NSCs通过其自我更新能力得到有效扩增。此外，我们使用四种重编程因子（Oct4、Sox2、Klf4和cMyc）直接从小鼠和人成纤维细胞诱导出NSCs，而无需对诱导多能干细胞（iPSCs）进行克隆分离。由于这些NSCs迅速发育成成熟的生成神经胶质细胞的神经干细胞，我们能够纯化这些快速分化的NSCs，而不会受到抗分化多能细胞的污染。这些方法将有助于高通量筛选散发性神经退行性疾病来源的体细胞诱导产生的神经细胞中出现的表型。
