Dental and Craniofacial Research Institute, UCLA, Los Angeles, CA, USA.
Stem Cells Transl Med. 2012 Sep;1(9):673-84. doi: 10.5966/sctm.2012-0053. Epub 2012 Sep 5.
Adipose tissue is an ideal mesenchymal stem cell (MSC) source, as it is dispensable and accessible with minimal morbidity. However, the stromal vascular fraction (SVF) of adipose tissue is a heterogeneous cell population, which has disadvantages for tissue regeneration. In the present study, we prospectively purified human perivascular stem cells (PSCs) from n = 60 samples of human lipoaspirate and documented their frequency, viability, and variation with patient demographics. PSCs are a fluorescence-activated cell sorting-sorted population composed of pericytes (CD45-, CD146+, CD34-) and adventitial cells (CD45-, CD146-, CD34+), each of which we have previously reported to have properties of MSCs. Here, we found that PSCs make up, on average, 43.2% of SVF from human lipoaspirate (19.5% pericytes and 23.8% adventitial cells). These numbers were minimally changed by age, gender, or body mass index of the patient or by length of refrigerated storage time between liposuction and processing. In a previous publication, we observed that human PSCs (hPSCs) formed significantly more bone in vivo in comparison with unsorted human SVF (hSVF) in an intramuscular implantation model. We now extend this finding to a bone injury model, observing that purified hPSCs led to significantly greater healing of mouse critical-size calvarial defects than hSVF (60.9% healing as opposed to 15.4% healing at 2 weeks postoperative by microcomputed tomography analysis). These studies suggest that adipose-derived hPSCs are a new cell source for future efforts in skeletal regenerative medicine. Moreover, hPSCs are a stem cell-based therapeutic that is readily approvable by the U.S. Food and Drug Administration, with potentially increased safety, purity, identity, potency, and efficacy.
脂肪组织是间充质干细胞（MSC）的理想来源，因为它是可有可无的，并且可以通过最小的发病率获得。然而，脂肪组织的基质血管部分（SVF）是一种异质细胞群体，这对组织再生有不利影响。在本研究中，我们前瞻性地从 n = 60 个人体脂肪抽吸样本中纯化了人血管周干细胞（PSCs），并记录了它们的频率、活力及其随患者人口统计学特征的变化。PSCs 是一种荧光激活细胞分选（FACS）分选的群体，由周细胞（CD45-、CD146+、CD34-）和外膜细胞（CD45-、CD146-、CD34+）组成，我们之前曾报道过这些细胞具有 MSC 的特性。在这里，我们发现 PSCs 平均占人体脂肪抽吸 SVF 的 43.2%（19.5%的周细胞和 23.8%的外膜细胞）。这些数字受患者年龄、性别、体重指数或脂肪抽吸与处理之间冷藏储存时间长短的影响很小。在之前的一篇论文中，我们观察到与未分选的人 SVF（hSVF）相比，人 PSCs（hPSCs）在肌肉内植入模型中体内形成的骨明显更多。我们现在将这一发现扩展到骨损伤模型，观察到纯化的 hPSCs 导致小鼠临界大小颅骨缺损的愈合明显好于 hSVF（通过微计算机断层扫描分析，术后 2 周时 hPSCs 组的愈合率为 60.9%，而 hSVF 组的愈合率为 15.4%）。这些研究表明，脂肪来源的 hPSCs 是骨骼再生医学未来努力的新细胞来源。此外，hPSCs 是一种基于干细胞的治疗方法，很容易被美国食品和药物管理局批准，具有潜在的更高安全性、纯度、身份、效力和疗效。
