Varga Ján, Tóth Štefan, Tóth Štefan, Tomečková Vladimíra, Gregová Kristína, Veselá Jarmila
2nd Department of Obstetrics and Gynecology, PJ Šafárik University and L Pasteur University Hospital, Košice, Slovak Republic.
Acta Biochim Pol. 2012;59(4):631-8. Epub 2012 Nov 30.
Questions regarding functional markers characterizing injured intestines remain unanswered. Brush border disaccharidases are crucial for the functioning of the intestines.
The study was designed to assess changes in disaccharidase activity (DA) following intestinal injury and to compare them with morphological changes.
Wistar rats, randomly divided into six experimental groups (each n = 6), were subjected to different ischemic/reperfusion injury. One-hour mesenteric ischemia followed by reperfusion for 0, 1, 2, 4, 12 or 24 hours was induced. As a control group sham-operated animals were used (n = 6). Intestine morphology was evaluated using histopathological injury index (HII) and goblet cell (GC) detection. DA (sucrase and maltase) was studied in mucosal scrape or in entire intestinal wall samples.
Moderate morphological damage (HII, GC) after mesenteric ischemia was detected. Deepening of the injury was found during reperfusion with a maximum after two hours. Improved morphology with longer reperfusion confirmed reversible damage with almost normal mucosal structure after 24 hours of reperfusion. Similar pattern was observed when DA was measured. The lowest activity was detected after 2 hours of reperfusion followed by increasing activity in the subsequent reperfusion periods. Physiological values after 24 hours of reperfusion were seen only in samples of entire intestinal wall.
Significant changes in intestinal DA were observed after intestinal ischemia/reperfusion injury. A similar pattern was seen for morphological characteristics. Although based on microscopic survey the intestine seems to be fairly regenerated, some functional limitation is expected to persist.
关于表征受损肠道的功能标志物的问题仍未得到解答。刷状缘双糖酶对肠道功能至关重要。
本研究旨在评估肠道损伤后双糖酶活性（DA）的变化，并将其与形态学变化进行比较。
将Wistar大鼠随机分为六个实验组（每组n = 6），使其遭受不同的缺血/再灌注损伤。诱导肠系膜缺血1小时，然后分别再灌注0、1、2、4、12或24小时。以假手术动物作为对照组（n = 6）。使用组织病理学损伤指数（HII）和杯状细胞（GC）检测评估肠道形态。在黏膜刮片或整个肠壁样本中研究DA（蔗糖酶和麦芽糖酶）。
检测到肠系膜缺血后出现中度形态学损伤（HII、GC）。再灌注期间损伤加重，两小时后达到最大值。随着再灌注时间延长，形态有所改善，表明损伤具有可逆性，再灌注24小时后黏膜结构几乎恢复正常。测量DA时观察到类似模式。再灌注2小时后检测到最低活性，随后在后续再灌注期活性增加。仅在整个肠壁样本中观察到再灌注24小时后的生理值。
肠道缺血/再灌注损伤后观察到肠道DA有显著变化。形态学特征也呈现类似模式。尽管基于显微镜检查肠道似乎已相当程度再生，但预计仍会存在一些功能限制。
