Arlow F L, Walczak S M, Moshier J A, Pietruk T, Majumdar A P
Veterans Administration Medical Center, Allen Park, MI 48101.
Life Sci. 1990;46(11):777-84. doi: 10.1016/0024-3205(90)90065-y.
An organ culture system was utilized to examine the effect of gastrin (G-17-I) and epidermal growth factor (EGF) on colonic mucosal ornithine decarboxylase (ODC) activity, and the expression of the ODC gene. Exposure of colonic mucosal explants to either gastrin or EGF (50-500 ng/ml) for only 4 h resulted in a profound stimulation (150-600%) in ODC activity over the basal level. These increases were essentially abolished by difluoromethylornithine (DFMO; 2 nmol/ml) or CaCl2 (2 umol/ml). Gastrin also activated the ODC gene in the colonic mucosa as evidenced by increased steady-state ODC mRNA levels in the colonic mucosal explants after 4 h exposure to the hormone, when compared with the controls. It is concluded that colonic mucosal ODC is responsive to both gastrin and EGF.
利用器官培养系统研究胃泌素（G-17-I）和表皮生长因子（EGF）对结肠黏膜鸟氨酸脱羧酶（ODC）活性及ODC基因表达的影响。将结肠黏膜外植体暴露于胃泌素或EGF（50 - 500 ng/ml）仅4小时，即可导致ODC活性比基础水平显著升高（150 - 600%）。二氟甲基鸟氨酸（DFMO；2 nmol/ml）或氯化钙（2 μmol/ml）可基本消除这些升高。与对照组相比，暴露于该激素4小时后，结肠黏膜外植体中ODC mRNA稳态水平升高，证明胃泌素也激活了结肠黏膜中的ODC基因。结论是结肠黏膜ODC对胃泌素和EGF均有反应。
