胃泌素和表皮生长因子对大鼠结肠外植体中鸟氨酸脱羧酶的诱导作用。

Gastrin and epidermal growth factor induction of ornithine decarboxylase in rat colonic explants.

作者信息

Arlow F L, Walczak S M, Moshier J A, Pietruk T, Majumdar A P

机构信息

Veterans Administration Medical Center, Allen Park, MI 48101.

出版信息

Life Sci. 1990;46(11):777-84. doi: 10.1016/0024-3205(90)90065-y.

DOI:10.1016/0024-3205(90)90065-y

PMID:2319906

Abstract

An organ culture system was utilized to examine the effect of gastrin (G-17-I) and epidermal growth factor (EGF) on colonic mucosal ornithine decarboxylase (ODC) activity, and the expression of the ODC gene. Exposure of colonic mucosal explants to either gastrin or EGF (50-500 ng/ml) for only 4 h resulted in a profound stimulation (150-600%) in ODC activity over the basal level. These increases were essentially abolished by difluoromethylornithine (DFMO; 2 nmol/ml) or CaCl2 (2 umol/ml). Gastrin also activated the ODC gene in the colonic mucosa as evidenced by increased steady-state ODC mRNA levels in the colonic mucosal explants after 4 h exposure to the hormone, when compared with the controls. It is concluded that colonic mucosal ODC is responsive to both gastrin and EGF.

摘要

利用器官培养系统研究胃泌素（G-17-I）和表皮生长因子（EGF）对结肠黏膜鸟氨酸脱羧酶（ODC）活性及ODC基因表达的影响。将结肠黏膜外植体暴露于胃泌素或EGF（50 - 500 ng/ml）仅4小时，即可导致ODC活性比基础水平显著升高（150 - 600%）。二氟甲基鸟氨酸（DFMO；2 nmol/ml）或氯化钙（2 μmol/ml）可基本消除这些升高。与对照组相比，暴露于该激素4小时后，结肠黏膜外植体中ODC mRNA稳态水平升高，证明胃泌素也激活了结肠黏膜中的ODC基因。结论是结肠黏膜ODC对胃泌素和EGF均有反应。