Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic.
Anal Chem. 2013 Jan 2;85(1):327-33. doi: 10.1021/ac302849q. Epub 2012 Dec 14.
The prospects of near- and supercritical water for treatment of the inner surfaces of fused silica capillaries have been tested employing an in-lab-assembled apparatus. Unlike all other agents used for the purpose, water cannot introduce any undesirable heteroatoms to the treated surface. Theoretical background for this work comes from the well-known fact that water near its critical point can solubilize silica. The results show that depending on the temperature, water flow rate, flow mode, and exposure time, high-temperature water has wide-ranging effects on both the surface roughness and the internal diameter profile along the length of the treated capillary. By judicious selection of the operating conditions, tapered capillaries of various profiles for applications in electromigration techniques can be prepared with relatively high reproducibility. The water-treated fused silica capillaries with uniform internal diameter appear to be useful for preparation of monolithic silica capillary columns.
采用实验室组装的设备，测试了近临界和超临界水对熔融石英毛细管内表面处理的前景。与其他用于此目的的试剂不同，水不会向处理后的表面引入任何不需要的杂原子。这项工作的理论背景来自于众所周知的事实，即在其临界点附近的水可以溶解二氧化硅。结果表明，取决于温度、水流量、流动模式和暴露时间，高温水对处理后的毛细管长度上的表面粗糙度和内径分布有广泛的影响。通过合理选择操作条件，可以以相对较高的重现性制备用于电迁移技术的各种轮廓的锥形毛细管。具有均匀内径的水处理熔融石英毛细管似乎可用于制备整体式硅胶毛细管柱。
