Favor J, Neuhäuser-Klaus A, Ehling U H
GSF-Institut für Säugetiergenetik, Neuherberg, F.R.G.
Mutat Res. 1990 Apr;229(2):105-14. doi: 10.1016/0027-5107(90)90084-h.
The frequency of dominant cataract and recessive specific-locus mutations and mutation mosaics was determined in F1 mice derived from post-spermatogonial germ-cell stage treatment with 2 X 80, 160 or 250 mg/kg ethylnitrosourea. A total of 5 dominant cataract mutations, 3 dominant cataract mutation mosaics, 1 specific-locus mutation and 9 specific-locus mutation mosaics were recovered in 15,542 screened F1 offspring. Results indicate that ethylnitrosourea treatment increases the mutation rate of dominant cataract and recessive specific-locus alleles in post-spermatogonial germ-cell stages of the mouse and that the mutations occur mainly as mosaics. Genetic confirmation of newly induced mutations occurring as mosaics is more problematical for induced recessive alleles than for induced dominant alleles and should be considered when evaluating such mutagenicity results.
在用2×80、160或250mg/kg乙基亚硝基脲对精原细胞后阶段生殖细胞进行处理所获得的F1代小鼠中，测定了显性白内障和隐性特定位点突变以及突变嵌合体的频率。在15542只经过筛选的F1代后代中，共发现了5个显性白内障突变、3个显性白内障突变嵌合体、1个特定位点突变和9个特定位点突变嵌合体。结果表明，乙基亚硝基脲处理会增加小鼠精原细胞后阶段生殖细胞中显性白内障和隐性特定位点等位基因的突变率，且这些突变主要以嵌合体形式出现。对于诱导产生的隐性等位基因而言，对以嵌合体形式出现的新诱导突变进行遗传确认比诱导产生的显性等位基因更具问题，在评估此类致突变性结果时应予以考虑。
