Stewart G J, Fitzgerald J W
Can J Microbiol. 1979 Sep;25(9):1008-14. doi: 10.1139/m79-155.
In the absence of added Mg2+, alkylsulfatase induction in resting cells of Pseudomonas aeruginosa was inhibited 17% by exogenous 0.05 mM UTP. Under these conditions, the cells converted UTP to ATP and rapid degradation of these nucleotides did not occur. In the presence of 0.73 mM Mg2+, 0.05 mM UTP repressed the synthesis of the enzyme by 71%. Under these conditions, the cells rapidly degraded both ATP derived from UTP as well as residual UTP. In the presence of Mg2+ and 0.1 mM UTP, full repression of alkylsulfatase formation occurred whereas Mg2+-depleted cell suspensions were still capable of synthesizing 47% of the enzyme under these conditions compared with control levels. The inhibition of alkylsulfatase induction was highly specific for UTP. Some inhibition was observed with exogenous uracil, uridine, and pyrophosophate but only at concentrations greater than 1.0 mM. Exogenous UMP and UDP (2mM) had no effect.
在没有添加Mg2+的情况下，铜绿假单胞菌静息细胞中烷基硫酸酯酶的诱导受到外源性0.05 mM UTP的17%抑制。在这些条件下，细胞将UTP转化为ATP，并且这些核苷酸没有快速降解。在存在0.73 mM Mg2+的情况下，0.05 mM UTP将该酶的合成抑制了71%。在这些条件下，细胞迅速降解了源自UTP的ATP以及残留的UTP。在存在Mg2+和0.1 mM UTP的情况下，烷基硫酸酯酶的形成完全受到抑制，而与对照水平相比，在这些条件下，缺乏Mg2+的细胞悬浮液仍能够合成47%的该酶。烷基硫酸酯酶诱导的抑制对UTP具有高度特异性。用外源性尿嘧啶、尿苷和焦磷酸观察到一些抑制作用，但仅在浓度大于1.0 mM时。外源性UMP和UDP（2 mM）没有作用。
