College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal University, Wuhu 241000, PR China.
Talanta. 2013 Jan 15;103:75-80. doi: 10.1016/j.talanta.2012.10.008. Epub 2012 Oct 12.
In the present work, sensitive and stable sandwiched electrochemical immunosensing strategies with graphene (Gr)/gold nanoparticles (GNP) composite as the immobilization platform and ferrocene (Fc) derivatives as labels were proposed. Under the optimal choice and the proposed immunosensing strategy, with 1,1'-ferrocenedicarboxylic acid label, the human IgG could be detected specifically with a low detection limit of 0.4 ng/mL and a wide linear dynamic range from 1 to 300 ng/mL. Meanwhile, the electrochemical behavior of three different ferrocene derivatives labels was studied.
在本工作中，提出了一种基于石墨烯（Gr）/金纳米粒子（GNP）复合材料作为固定化平台，以二茂铁（Fc）衍生物作为标记物的灵敏、稳定的夹心型电化学免疫传感策略。在最优选择和所提出的免疫传感策略下，以 1,1'-二茂铁二甲酸为标记物，可特异性检测人 IgG，检测限低至 0.4ng/mL，线性动态范围为 1-300ng/mL。同时，研究了三种不同的二茂铁衍生物标记物的电化学行为。
