Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences/College of Biotechnology, University of Santiago de Compostela, Rúa Carballo Calero s/n, Campus Lugo, E-27002 Lugo, Spain.
Food Microbiol. 2013 Apr;33(2):235-42. doi: 10.1016/j.fm.2012.09.022. Epub 2012 Oct 11.
The Bacillus genus includes species such as Bacillus cereus, Bacillus licheniformis and Bacillus subtilis, some of which may be pathogenic or causative agents in the spoilage of food products. The main goal of this work was to apply matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass fingerprinting to the classification of these Bacillus species. Genetic analyses were also compared to phyloproteomic analyses. A collection of 57 Bacillus strains isolated from fresh and processed food and from culture collections were studied and their mass spectra compiled. The resulting mass fingerprints were compared and characteristic peaks at the strain and species levels were assigned. The results showed that MALDI-TOF was a good complementary approach to 16S rRNA sequencing and even a more powerful tool in the accurate classification of Bacillus species, especially for differentiating B. subtilis and B. cereus from Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively. MALDI-TOF was also found to provide valuable information at both intra- and interspecies levels in the Bacillus species studied.
芽孢杆菌属包括蜡样芽孢杆菌、地衣芽孢杆菌和枯草芽孢杆菌等物种，其中一些可能是导致食品变质的病原体或致病因子。本工作的主要目的是应用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱指纹图谱对这些芽孢杆菌进行分类。同时还比较了遗传分析和系统发育蛋白质组学分析。研究了从新鲜和加工食品以及从培养物收集的 57 株芽孢杆菌菌株，并对其质谱进行了编译。对得到的质谱指纹图谱进行了比较，并在菌株和物种水平上分配了特征峰。结果表明，MALDI-TOF 是 16S rRNA 测序的良好补充方法，甚至是芽孢杆菌物种准确分类的更有力工具，特别是在区分枯草芽孢杆菌和蜡样芽孢杆菌与解淀粉芽孢杆菌和苏云金芽孢杆菌方面。MALDI-TOF 还在研究的芽孢杆菌物种中提供了在种内和种间水平的有价值的信息。
