Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Distrito Federal, Mexico.
Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Distrito Federal, Mexico.
Vet Microbiol. 2013 Mar 23;162(2-4):491-498. doi: 10.1016/j.vetmic.2012.10.037. Epub 2012 Nov 11.
Porcine rubulavirus is the etiological agent of blue eye disease in pigs. In boars, this virus causes orchitis and epididymitis and reduces seminal quality. The objective of this study was to determine the persistence of porcine rubulavirus in experimentally infected boars. Nine 12-month-old boars were infected with 5 ml of the PAC-3 strain of porcine rubulavirus at 1 × 10(5) TCID(50)/ml and held for 142 days post infection (DPI) to evaluate humoral immune response. The virus was isolated in cell cultures and detected by RT-PCR. Infection with porcine rubulavirus produced clinical signs beginning at 5 DPI. Necropsy results showed that 3 boars had lesions in the testicles and epididymes. Histological analysis showed the characteristic lesions in all infected boars. Porcine rubulavirus antibodies were detected in the second week post infection and increased significantly (P<0.05) over time. Isolation of the virus from semen was achieved between 5 DPI and 48 DPI and from the testicles and epididymes between 64 DPI and 142 DPI. Viral RNA was detected in the serum between 2 DPI and 64 DPI and in the semen until 142 DPI. These results confirm that the RNA of the porcine rubulavirus persists in the semen and that this virus remains in the reproductive tract for prolonged periods of infection. Semen of persistently infected boars, therefore, represents an important source of the virus and a risk factor for the spread of blue eye disease in swine populations.
猪 rubulavirus 是猪蓝眼病的病原体。在公猪中，这种病毒会引起睾丸炎和附睾炎，并降低精液质量。本研究的目的是确定实验感染公猪中猪 rubulavirus 的持续存在。将 5 ml 的 PAC-3 株猪 rubulavirus 以 1×10(5)TCID(50)/ml 的浓度感染 9 头 12 月龄公猪，并在感染后 142 天（DPI）评估体液免疫反应。通过细胞培养分离病毒并通过 RT-PCR 检测。感染猪 rubulavirus 后，从第 5 天开始出现临床症状。尸检结果表明，3 头公猪的睾丸和附睾有病变。组织学分析显示所有感染公猪均有特征性病变。感染后第二周检测到猪 rubulavirus 抗体，且随时间推移显著增加（P<0.05）。从 5 DPI 到 48 DPI 从精液中分离出病毒，从 64 DPI 到 142 DPI 从睾丸和附睾中分离出病毒。在 2 DPI 到 64 DPI 之间的血清中检测到病毒 RNA，在精液中检测到直到 142 DPI。这些结果证实，猪 rubulavirus 的 RNA 存在于精液中，该病毒在生殖道中持续存在很长时间。因此，持续感染的公猪的精液是病毒的重要来源，也是猪群中蓝眼病传播的危险因素。
