比较两种选择性培养基从环境表面回收艰难梭菌的效果。
Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces.
机构信息
Microbiology Department, Freeman Hospital, Newcastle upon Tyne, UK.
出版信息
J Hosp Infect. 2013 Feb;83(2):164-6. doi: 10.1016/j.jhin.2012.10.006. Epub 2012 Nov 30.
Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin-cycloserine-egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile. A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C. difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C. difficile was 87.6% compared with 26.6% for CCEY/L (P < 0.0001). chromID C. difficile performed significantly better than CCEY/L for the recovery of C. difficile from the environment.
两种培养基被比较，以评估它们在英国医院环境中分离艰难梭菌的能力。这两种培养基分别为头孢西丁-环丝氨酸-卵黄琼脂加溶菌酶（CCEY/L）和 chromID C. difficile。使用无菌海绵（Polywipes）对广泛的环境表面进行采样，并将这些海绵接种到两种培养基上。使用这两种培养基的组合，从 496 个采样点中的 105 个（21%）中回收了艰难梭菌。chromID C. difficile 的灵敏度为 87.6%，而 CCEY/L 为 26.6%（P < 0.0001）。chromID C. difficile 在从环境中回收艰难梭菌方面的表现明显优于 CCEY/L。
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